Biodistribution study of [61Cu]pyruvaldehyde-bis (N-4-methylthiosemicarbazone) in normal rats as a PET tracer

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE

Biodistribution study of [⁶¹Cu]pyruvaldehyde-bis (N-4-methylthiosemicarbazone) in normal rats as a PET tracer

Amir Reza JALILIAN，

Saeed SHANESAZZADEH，

Pejaman ROWSHANFARZAD，

Fatemeh BOLOURINOVIN，

Abbas MAJDABADI

Nuclear Science and Techniques

Vol.19, No.3

pp.159-164

Published in print 20 Jun 2008

DOI：10.1016/S1001-8042(08)60043-3

CLC：R817.4,O621.3+5

49700

[⁶¹Cu]-labeled pyruvaldehyde-bis(N-4-methylthiosemicarbazone) (⁶¹Cu-PTSM), a promising agent made for imaging blood perfusion, was produced via the ^natZn(p,x)⁶¹Cu nuclear reaction in a 30 MeV cyclotron, and separated by a two-step column chromatography method developed in our laboratory using a cation and an anion exchange resin. After 150 μA irradiation for 76 min, about 6.006 Ci of ⁶¹Cu²⁺ was obtained with a radiochemical separation yield of 95% and a radionuclidic purity of 99%. ⁶¹Cu-PTSM was prepared using an optimized method with in-house synthesized PTSM ligand for radiolabeling following quality control procedures using RTLC and HPLC. The tracer is mostly incorporated in heart, kidneys and brain compared to free copper cation as a control. These are in agreement with former reports. In conclusion, [⁶¹Cu]-PTSM was prepared at the radiopharmaceutical scales with high quality and is a potential PET tracer in the perfusion study of the heart, kidney, brain and tumors.

Copper-61PTSMBiodistributionRadiolabeling

References

[1]

Jalilian AR, Seyfi P, Afarideh H, et al. Int. J Appl Radiat Isot. 2000, 5: 545-555.

[2]

Jalilian AR, Afarideh H, Shafiee A, et al. J Pharm Pharmaceut. Sci. 2000; 23: 114-124.

[3]

Jalilian AR, Afarideh H, Shafiee A, et al. J Labelled Compd Radiopharm. 2001, 44: 11-21.

[4]

Tolmachev V, Lundqvist H, Einarsson L. Int J Appl Radiat Isot. 1998, 49: 79-81.

[5]

McCarthy DW, Bass LA, Cutler PD, et al. Nucl Med Biol. 1999, 26: 351-358.

[6]

Ma DF, Lu T, et al. Nucl Med Biol. 2002; 29: 91-105

[7]

Fukumura T, Okada K, Szelecsényi F, et al. Radiochimica Acta. 2004, 92: 209-214.

[8]

Lewis JS, Sharp TL, Laforest R, et al. J Nucl Med. 2001, 42: 655-661.

[9]

Rowshanfarzad P, Sabet M, Jalilian AR, et al. Applied Radiation and Isotopes, 2006, 64:1563-1573.

[10]

Jalilian AR, Sabet M, Rowshanfarzad P, et al. Journal of Radioanalytical and Nuclear Chemistry, 2006, 269: 147-154.

[11]

Jalilian AR, Rowshanfarzad P, Sabet M. Nukleonika, 2006, 51: 111-117.

[12]

Jalilian AR, Rowshanfarzad P, Sabet M, et al. Applied Radiation and Isotopes, 2006, 64: 337-341.

[13]

Green MA. Nucl Med Biol 1987, 14:59-61.

[14]

Marczenko Z. Spectrophotometric determination of elements, 4th Ed. Warsaw:Ellis Horwood Ltd., 1976:601-603.

[15]

Kostyniak PJ, Nakeeb SM, Schopp EM, et al. J Appl Toxicol, 1990, 10:417-421.

[16]

Shelton ME, Green MA, Mathias CJ, et al. Circulation, 1990, 82:990-997.

[17]

Young H, Carnochan P, Zweit J, et al. Eur J Nucl Med, 1994, 21:336-341.

[18]

Young H, Carnochan P, Zweit J, et al. J Nucl Biol Med, 1994, 38:89-91.

[19]

Wada K, Fujibayashi Y, Taniuchi H, et al. Nucl Med Biol, 1994, 21:613-617.

[20]

Jason SL, Laforest R, Buettner TL, et al. Proc Natl Acad Sci USA, 2001, 98:1206-1211.

[21]

Blann M, Bislinghoff J,

Code Alice/Livermore 91, Lawrence Livermore National Laboratory, Internal Report, UCID-19614

(1991).

Baidu Scholar

Google Scholar

[22]

Ziegler JF, Biersack JP, Littmark U. The stopping and range of ions in matter (SRIM Code). Version 2000 XX, 2000.

[23]

Weisberg A M, Gold plating, 9th Ed., ASM International, U.S.A, 1990:247.

[24]

Hilgers K, Stoll T, Skakun Y, et al. Appl Radiat Isot, 2003, 59:343-351.

[25]

Boothe TE, Tavano E, Munoz J, et al. J Label Comp Radiopharm, 1991, 30:108.

[26]

Gingras BA, Suprunchuk T, Bayley CH. Can J Chem, 1962, 40:1053-1057.

[27]

Marczenko Z. Spectrophotometric determination of elements, 4th Ed., Chichester: Ellis Horwood Ltd., 1976:369.

[28]

Wallhaus TR, Lacy J, Whang J, et al. J Nucl Med, 1998, 39:1958-1964.