Effect of 125I seeds and 103Pd stents on proliferation of vascular smooth muscle cells
This study aims at the theoretical and practical evidence for prevention of restenosis in vitro. Vascular smooth muscle cell (VSMC) model was established using adherent cell culture methods. The proliferation of VSMC was investigated by the cell counting method and 3H-TDR implementation test. The results are as follows. (1) For 125I-seeds, the inhibition rate was 29.3% at 74Bq (p<0.05), 35.2% at 148Bq (p<0.05) and 42.4% at 370Bq (p<0.05), For 103Pd-implanted stents, the inhibition rate was 14.7% at 4.44 MBq (p<0.05), 24.0% at 5.92 MBq (p<0.05) and 38.0% at 7.4 MBq (p<0.05), There was no significant difference between the blank tests and non-radioactive tests. (2) 48 hours exposure from 125I-seeds at 148 Bq or 103Pd-implanted stents at 7.4 MBq did not result in VSMC's morphological alteration, but that from 125I-seeds at 370Bq caused morphological changes, Both 125I-seeds and 103Pd-implanted stents inhibit the VSMC DNA synthesis in vitro. The inhibition effects are significantly related to their exposure duration and doses.
