An improved Coomassie Brilliant Blue (CBB R-250) staining to proteins in gels

ZHOU Zhi-Dong，

LIU Wang-Yi，

LI Min-Qian

Nuclear Science and Techniques

Vol.13, No.4

pp.251-258

Published in print 01 Nov 2002

CLC：Q503, Q51

25200

An improved CBB staining with higher sensitivity than that of the typical CBB staining was reported. The main improvement was using a fixing step of 25% trichloroacetic acid (TCA) before CBB staining. For most proteins studied, the sensitivity of the improved CBB staining was about twice as high as that of the typical method. For basic and low molecular weight proteins such as ribosomal proteins, the sensitivity of this improved staining method was about 3.5-28 times that of the typical method. It was speculated that the improved procedure would be suitable for exact quantitative analysis of proteins fractionated by SDS-PAGE, especially for basic and low molecular weight proteins. On the other hand, this new modified method might be also applied to multidisciplinary studies, such as biological researches and nuclear sciences.

Basic and low molecular weight proteinsCoomasie Brilliant Blue (CBB) R-250Ribosomal proteinsSDS-PAGE2-D electrophoresis

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