When individual microorganisms were used to release bound pesticide residues, it was ussually not take into full account whether the autoclaving method could lead to the release of bound residues. The soil containing bound ¹⁴C-chlorsulfuron residues was treated with an autoclave in this study. The results reveal that the bound ¹⁴C-chlorsulfuron residue can be released from the soil when treated with the autoclave and the release rate can be remarkably enhanced by adding water into the soil when subjected to such treatment. A TLC analysis showed that the released ¹⁴C-residue was one of the degraded products of ¹⁴C-chlorsulfuron. After derivatization and analysis using the GC-MS, the released ¹⁴C-residue was tentatively identified as 2-amino-4-hydroxyl-6-methyl-1,3,5-triazine.