Modified "DMC" technique for stretching DNA molecules

Modified "DMC" technique for stretching DNA molecules

LI Bin，

ZHANG Yi，

OUYANG Zhenqian，

HU Jun，

HUANG Yibo，

LI Minqian

Nuclear Science and Techniques

pp.134-136

Published in print 01 Aug 1999

55400

A modified "dynamic molecular combing" (DMC) technique used for stretching double-stranded DNA is reported. DNA molecules were stretched on the silanized mica surface by this technique, its speed being precisely controlled with a computer. This approach combined the precise DNA stretching method with high resolution AFM imaging at nanometer scale, thus making it useful for DNA alignment manipulation and subsequent gene research.

Atomic force microscopyDynamic molecular combingStretching DNA molecules

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