Radioimmunoassay of pepsinogens Ⅰ and Ⅱ in human serum

Xiao Zhi-Jian，

Jiang Meng-Jun，

Huang Xu-Quan

Nuclear Science and Techniques

Vol.9, No.1

pp.33-35

Published in print 01 Feb 1998

30500

Radioimmunoassay (RIA) for pepsinogens Ⅰ and II (PG Ⅰ and Ⅱ) in serum is developed by using the purified PG Ⅰ and Ⅱ from human gastric mucosa. The assay range was 8~256μg/L for PG Ⅰ and 2~64μg/L for PG Ⅱ, the sensitivity was 1.3 and 0.6µg/L, respec-tively. The reproducibilities of PG Ⅰ-RIA (106.9%) and PG Ⅱ-RIA (106.7%) are quite well. The within-and between-assay coefficient of variation (CV) of each RIA was 5.1% and 6.3% for PG Ⅰ, 7.2% and 8.9% for PG Ⅱ. The serum PG Ⅰ level in healthy volunteer was 60.41±14.98µg/L (mean±SD), significantly higher than the serum PG Ⅱ level which was 20.70±9.64µg/L. There-fore, the PG Ⅰ-RIA and PG Ⅱ-RIA are useful tools in studying the relation between serum pepsinogen levels and various peptic disorders.

PepsinogenRadioimmunoassayStomach cancerTumor marker125I

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