Comparison of DNA double-strand breaks induced by 16O8+ in deproteinized DNA and intact cells

Comparison of DNA double-strand breaks induced by ¹⁶O⁸⁺ in deproteinized DNA and intact cells

Zhou Guang-Ming，

Wei Zeng-Quan，

Li Wen-Jian，

Gao Qing-Xiang，

He Jing，

Li Qiang，

Wang Jü-Fang

Nuclear Science and Techniques

Vol.9, No.4

pp.235-238

Published in print 01 Nov 1998

20700

The yield of DNA double-strand breaks (DSBs) is sure to be influenced by the environment around DNA molecule. Inverse pulsed-field gel electrophoresis (PIGE) has been applied to compare the sensitivity of B16 cells and their DNA in DSBs induced by 75 MeV/u ¹⁶O⁸⁺ beam. Results show that the percentages of DNA released from the plug(PR) in both kinds of the samples increase with the dose and approach a similar quasi-threshold of about 81%. A simple new equation was presented to calculate the break level of DNA molecules. Within a certain dose, the relationship between the break level and the dose is linear. The yield of DSBs in deproteinized DNA was 1.11 DSBs/100 Mbp/Gy, while that in intact cells was 0.60DSBs/100Mbp/Gy. It is testified that deproteinized DNA is more sensitive to oxygen ions irradiation than intact cells.

DNA double-strand breaks75 MeV/u 16O8+ beamMelanomas cells

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