ACTIVE CALCIUM TRANSPORT IN PLASMA MEMBRANE VESICLES FROM DEVELOPING COTYLEDONS OF COMMON BEAN

Huang Jianzhong，

Chen Ziyuan

Nuclear Science and Techniques

pp.31-36

Published in print 01 Feb 1995

22400

Plasma membrane vesicles were prepared from the developing cotyledons of common bean (Phaseolus vulgaris L cv Diyundou) by aqueous two-phase partitioning and characterized as to their purity by assaying marker enzymes for other membranes. The putative plasma membrane fraction was minimally contaminated by membranes other than plasma membrane and hence was of high purity. It exhibited a Ca²⁺-dependent ATPase activity, which was inhibited by 1 µmol/L EB and promoted by calcium ionophore A23187. Such an activity was responsible for the observed ATP-dependent ⁴⁵Ca²⁺ uptake into inside-out plasma membrane vesicles. This process was stimulated by 0.6 µmol/L CaM and 20 µmol/L IAA but inhibited by 2 µmol/L ABA and abolished by A23187. Possible role of cytoplasmic Ca²⁺ in mediating phytohormones activity is discussed.

45Ca2+ uptakePlasma membrane vesiclesPhytohormonesDeveloping cotyledons of common beanTracer techniques

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