NASOPHARYNGEAL CARCINOMA RADIOSENSITIVITY PREDICTION BY CYTOKINESIS-BLOCK MICRONUCLEUS ASSAY

Yang Xing，

Shi Jianhui，

Cheng Wenying

Nuclear Science and Techniques

Vol.6, No.1

pp.47-50

Published in print 01 Feb 1995

29800

Cytokinesis-block micronucleus method is used to evaluate the radiosensitivity of a nasopharyngeal carcinoma cell line (CNE-1) and biopsies obtained from 31 patients with nasopharyngeal carcinoma. The number of micronuclei increases with the radiation dose. A good correlation was found between the radiosensitivity determined by the micro-nucleus assay and that measured by the colony-forming assay in CNE-1 cell line (r=-0.998). Moreover, the results of micronucleus assay for tumor cells from biopsies of patients with primary carcinoma look promising for the prediction of tumor radiosensitivity. These results are encouraging but need to be confirmed with a larger number of patients with a longer follow-up.

Micronucleus assayRadiosensitivityNasopharyngeal carcinoma

REFERENCES

Fenech M, Morley A A. Mutation Res, 1985; 147: 29

Weissenborn U, Streffer C. Int J Radiat Biol, 1990; 59: 373

Shibamoto Y, Streffer C, Fuhrmann C et al. Radiat Res, 1991; 128: 293

Streffer C. Br J Radiol, 1992; 24(Suppl): 70

Ban S, Cologene J B, Fujita S et al. Radiat Res, 1993; 134: 170

Tang Weisheng. Foreign Med-Radiat Med and Nucl Med (in Chinese), 1991; 15: 10

Fenech M, Morley A A. Mutation Res, 1990; 57: 373

Qisano . Clinical Carcinoma (Japan), 1989; 35: 1566

Zamboglou N, Streffer C, Schnabel T H et al. Int J Radiat Oncol Biol Phys, 1992; 25: 15

Wandl E O, Ono K, Kain R et al. Int J Radiat Biol, 1989; 56: 771