A NEW APPROACH TO THE CURVE FITTING OF IN VITRO RADIOASSAYS BASED ON THE MASS ACTION LOW

A NEW APPROACH TO THE CURVE FITTING OF IN VITRO RADIOASSAYS BASED ON THE MASS ACTION LOW 1 first-author Shanghai Second Medical University, Shanghai 200025, China Shanghai Second Medical University, Shanghai 200025, China A new curve fitting method with its mathematical models derived from the mass action law is presented which is applicable to several in vitro radioassays including RIA and RBA. Experiments revealed that the robustness of this method is better than the conventional methods like Woolf plot in RBA and 4- parameter logistic plot in RIA. However, the robustness of this method is only relative: in some cases of RIA and RBA, the bias of the results may still be too large to be acceptable. Further improvement is expected to be studied. Vitro radioassays Curve fitting Mathematical models Mass action law REFERENCES D. Rodbard and R.L. Tacey , Anal. Biochem. , 90 ( 1978 ), 13 . Anal. Biochem. T.A Wilkins et al ., Radioimmunoassay and related procedures in medicine , vol 1 , IAEA , Berlin , 1977 , p. 399 . Radioimmunoassay and related procedures in medicine P.R. Edwards and R.P. Ekins , ibid ., IAEA , Vienna , 1982 , p. 423 . P.R. Edwards . The WHO immunoassay program (Version 5.2) , London , 1985 . The WHO immunoassay program (Version 5.2) D. Rodbard and D.M. Hutt , Radioimmunoassay and related procedures in medicine , IAEA , Vienna , 1974 , p. 165 . Radioimmunoassay and related procedures in medicine 1990-08-01 2021-02-05 1990 Nuclear Science and Techniques 3 1