SYNTHESIS OF (³H-METHYL) TRINITROTOLUENE AND ITS APPLICATION TO TOXICOLOGICAL STUDY

SYNTHESIS OF ( 3 H-METHYL) TRINITROTOLUENE AND ITS APPLICATION TO TOXICOLOGICAL STUDY 1 first-author 1 1 1 1 2 2 2 2 Shanghai Institute of Nuclear Research, Academia Sinica, Shanghai 201800, China Shanghai Institute of Nuclear Research, Academia Sinica, Shanghai 201800, China Department of Occupational Health, Shanghai Medical University, Shanghai 200032, China Department of Occupational Health, Shanghai Medical University, Shanghai 200032, China In this paper, ( 3 H- methyl) toluene was prepared by catalysed halogen- tritium substitution method from benzyl bromide, then it was nitrated to produce ( 3 H- methyl) trinitrotoluene. The tritiated product was purified by thin- layer chromatography. At last, the pure 3 H- TNT was obtained with specific radioactivity of 3.77 GBq/mmol. Radiochemical purity was over 98% and the ultraviolet absorption spectrum of tritiated TNT was conformed with that of standard sample. Using 3 H- TNT as a tracer, its toxicokinetics was studied in rats. The results showed that the toxicokinetics characteristics of TNT were quickly absorbed into the blood, V d > 2L/kg.h, long T 1/2 β and fixed accumulation with four routes of administration, TNT and its metabolites were mainly excreted by the urine. The half- life of TNT in the urine were 11- 24h. A trace of radioactivity of 3 H- TNT and its metabolites could be detected in the urine on 7th day after administration (9.25 × 10 6 Bq/kg). ( 3 H- methyl) toluene ( 3 H- methyl) trinitrotoluene Catalysed halogen- tritium substitution method Toxicology Toxicokinetics REFERENCES B.S. Levine et al ., Toxicology , 32 ( 1984 ), 253 . Toxicology B.H. Woollen et al ., Bri. J. Ind. Med. , 43 ( 1986 ), 465 . Bri. J. Ind. Med. Feng Kang et al ., The numerical method ; National Defence Industry Press , Beijing , 1987 , p. 154 - 156 . The numerical method Liu Xiaochang et al ., The introduction of pharmacokinetics , Chinese Technology Press , Beijing , 1984 , p. 290 . The introduction of pharmacokinetics Fan Guoping et al ., Nucl. Tech. (Shanghai) , 10 ( 1987 ), 4 : 53 . Nucl. Tech. (Shanghai) 1991-02-01 2021-02-05 1991 Nuclear Science and Techniques 1 2