INDUCTION OF MUTAGENIC EFFECT IN SOMATIC AND GERM CELLS BY ENRICHED URANIUM

INDUCTION OF MUTAGENIC EFFECT IN SOMATIC AND GERM CELLS BY ENRICHED URANIUM 1 first-author 1 1 1 Suzhou Medical College, Suzhou 215007, China Suzhou Medical College, Suzhou 215007, China Enriched uranium(UO 2 F 2 ) accumulated in organism could cause chromosome aberrations in somatic cells, its rates on bone marrow cells were elevated when the dose of 235 UO 2 F 2 was increased. Among the types of induced aberrations, chromatid breakage was predominant, accompanied with a few chromosome breakage and translocation. At the same time mitosis index of metaphase cells was depressed. Chromatid delation and chromatid exchange were induced in peripheral blood lymphocytes. The important type of aberrations in spermatogonia was break. For primary spermatocytes the most significant aberration was multivalents which resulted either from chromatid interchanges or reciprocal translocations. 235 UO 2 F 2 could result in DNA breakage in germ cells. The sensitivity of germ cells at various stages to 235 UO 2 F 2 . was different. At 12 d after exposure the amount of sperm DNA eluted reached the peak. When the treating time was fixed, elution of sperm DNA from treated animals increased with the increasing doses. 235 UO 2 F 2 could also result in sperm abnormalities. Especially at 13 to 36 d after treatment the rates of sperm abnormalities were significantly elevated. Enriched uranium Somatic cells Germ cells Chromosome aberrations DNA Mutagenic effect REFERENCES Zhu Shoupeng . Radiotoxicology (in Chinese) . Beijing : Atomic Energy Press , 1983 , 78 - 89 . Radiotoxicology (in Chinese) Ma Zhongzi . Handbook of radioactive nuclides . Beijing : Atomic Energy Press , 1979 , 60 . Handbook of radioactive nuclides Zhu Shoupeng , Zheng Siying , Wang Liuyi . Chinese Journal of Preventive Medicine , 1989 , 23 ( 3 ): 166 . Chinese Journal of Preventive Medicine Zhu Shoupeng , Su Kunyuan , Chi Shamdong . Nuclear Protection (in Chinese) , 1979 , ( 2 ): 24 . Nuclear Protection (in Chinese) Zhu Shoupeng , Wang Chongdao , Wang Guolin . Acta Pharmacologica Sinica , 1984 , 5 : 214 . Acta Pharmacologica Sinica Zhu Shoupeng , Zhao Jingyong , Su Kunyuan . Nuclear Techniques(in Chinese) , 1980 , 13 ( 4 ): 47 . Nuclear Techniques(in Chinese) Evans P W . Cytogenetics , 1964 , 3 : 289 . Cytogenetics Ito Y . Mutation Research , 1988 , 206 : 55 . Mutation Research Watson W A F , Petrie D E , Galloway D E . Mutation Research , 1976 , 38 : 71 . Mutation Research Preston R J , Au W , Bender M A . Mutation Research , 1981 , 97 : 143 . Mutation Research Sega G A , Sotomayer R E . In: de Serres F J , Hollander A eds. Chemical mutagens: Principles and methods for detection . New York : Plenum Press , 1982 . Vol. 7 , 421 - 429 . Chemical mutagens: Principles and methods for detection Zhu Shoupeng , Zhang Lansheng . Isotopic tracers in medicine (in Chinese) . Beijing : Atomic Energy Press , 1989 , 77 - 81 Isotopic tracers in medicine (in Chinese) The Subject Supported by National Natural Science Foundation of China 1993-02-01 2021-02-05 1993 Nuclear Science and Techniques 1 4