Rhenium labeled McAb-SZ39 and colloid

Rhenium labeled McAb-SZ39 and colloid 1 first-author 1 1 1 Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800 Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800 Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared. Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with <math><mrow><msup><mrow/><mrow><mn>186</mn></mrow></msup><mi>Re</mi><msubsup><mi>O</mi><mn>4</mn><mo>−</mo></msubsup></mrow></math> . When ascorbic acid was used as reduction agent of antibody and the reduction of <math><mrow><msup><mrow/><mrow><mn>186</mn></mrow></msup><mi>Re</mi><msubsup><mi>O</mi><mn>4</mn><mo>−</mo></msubsup></mrow></math> was finished in sodium gluconate solution (pH=2.54), the labeling efficiency was 96.2%. After purification, the labeled products were tested in respect of immunoreactivity, in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice. <math><mrow><msup><mrow/><mrow><mn>188</mn></mrow></msup><mi>Re</mi><msubsup><mi>O</mi><mn>4</mn><mo>−</mo></msubsup></mrow></math> labeled colloid was synthesized by using a two-step method. The size of radiocolloid particles was around 2~5μm, and the radiochemical yield was over 95%. Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188 Re-colloid and 188 Re-McAb-SZ39 was 90% and 65%, respectively. Radiopharmaceuticals <math><mrow><msup><mrow/><mrow><mn>186</mn></mrow></msup><mi>Re</mi><msubsup><mi>O</mi><mn>4</mn><mo>−</mo></msubsup></mrow></math> <math><mrow><msup><mrow/><mrow><mn>188</mn></mrow></msup><mi>Re</mi><msubsup><mi>O</mi><mn>4</mn><mo>−</mo></msubsup></mrow></math> Label McAb-SZ39 Colloid References Zhu J H , Du Z W , Huang Q et al . J Suzhou Med Col (in Chinese) , 1988 , 8 : 276 ~ 281 J Suzhou Med Col (in Chinese) Pressman D , Keighley G . J Immunol , 1948 , 59 : 141 ~ 146 J Immunol Goldrosen M H , Vanderheyden J L. Fritzberg A R et al . Cancer Res , 1990 , 50 : 7973 ~ 7978 Cancer Res Vanderheyden J L , Gofinch S. Srinivasan A et al . J Nucl Med , 1989 . 30 : 793 ~ 798 J Nucl Med John E , Thakur M J , Defulvio M R et al . J Nucl Med . 1993 , 34 : 260 ~ 267 J Nucl Med Griffiths G L. Gddenberg D M. Hansen H J et al . Cancer Res , 1994 , 73 : 767 ~ 768 Cancer Res Wang S J , Lin W Y , Hereh B T et al . Eur J Nucl Med , 1995 , 22 : 505 ~ 507 Eur J Nucl Med Liu Z L , Luo S Z , Dai T E . Isotope (in Chnese) , 1995 , 8 : 65 ~ 70 Isotope (in Chnese) 1999-09-01 1999-11-01 2021-02-05 1999 Nuclear Science and Techniques 4 10