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Synchrotron FTIR spectroscopy reveals molecular changes of
Escherichia coli upon Cu2+exposure- Nuclear Science and Techniques Vol. 27, Issue 3, Article number: 56(2016)
- Affiliations：
1.Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
2.University of Chinese Academy of Science,Beijing100049, China
3.Shanghai Center for Bioinformation Technology, Shanghai Academy of Science &Technology; Center for Clinical and translational medicine, Shanghai Industrial Technology Institute, Shanghai 201203, China
- Author bio：
Correspondence address: lujunhong@sinap.ac.cn
- Funds：
DOI：10.1007/s41365-016-0067-9
CLC：
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Xiao-Juan Hu, Zhi-Xiao Liu, Ya-Di Wang, et al. Synchrotron FTIR spectroscopy reveals molecular changes of
Escherichia coli upon Cu2+exposure. [J]. Nuclear Science and Techniques 27(3):56(2016)Xiao-Juan Hu, Zhi-Xiao Liu, Ya-Di Wang, et al. Synchrotron FTIR spectroscopy reveals molecular changes of
Escherichia coli upon Cu2+exposure. [J]. Nuclear Science and Techniques 27(3):56(2016) DOI： 10.1007/s41365-016-0067-9.
摘要
Abstract
Copper ions (e.g. Cu,2+,) have outstanding antibacterial properties, but the exact mechanism is rather complex and not fully understood. In this work, synchrotron Fourier transform infrared (FTIR) spectroscopy was used as an analytical tool to investigate the CuCl,2,-induced biochemical changes in ,Escherichia coli,. Our spectral measurements indicated that this technique issensitive enough to detect changes in membrane lipids,nucleic acids, peptidoglycans and proteins of Cu,2+,-treated bacteria. Interestingly, for short-timetreated cells, the effects on phospholipids composition were clearly shown while no significant alterationsof proteins, nucleic acids and peptidoglycans were found. Quantitative Nano-Mechanics (PF-QNM) mode atomic force microscopy (AFM) confirmedthe changes of the topography and mechanical properties of bacteria upon the Cu,2+, exposure. This study demonstrated that FTIRspectroscopy combined with AFM can provide more comprehensive evaluation on the biochemical and mechanicalresponses of bacteria to copper.
关键词
Keywords
copper ionsAntibacterial effectEscherichia coliSynchrotronFTIRspectroscopyAtomic force microscopy
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