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A high-throughput screening method for breeding
Aspergillus niger with 12C6+ ion beam-improved cellulase- Nuclear Science and Techniques Vol. 28, Issue 1, Article number: 1(2017)
- Affiliations：
1.Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd, Lanzhou 730000, Gansu province, PR China
2.University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China
3.Lanzhou University, 222 South Tianshui Road, Lanzhou 730000, Gansu province, PR China
4.Gansu Agricultural University, Lanzhou 730000, Gansu province, PR China
- Author bio：
Corresponding author. E-mail address: wangsy@impcas.ac.cn
- Funds：
DOI：10.1007/s41365-016-0157-8
CLC：
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Bo-Ling Jiang, Shu-Yang Wang, Yu-Chen Wang, et al. A high-throughput screening method for breeding
Aspergillus niger with 12C6+ ion beam-improved cellulase. [J]. Nuclear Science and Techniques 28(1):1(2017)Bo-Ling Jiang, Shu-Yang Wang, Yu-Chen Wang, et al. A high-throughput screening method for breeding
Aspergillus niger with 12C6+ ion beam-improved cellulase. [J]. Nuclear Science and Techniques 28(1):1(2017) DOI： 10.1007/s41365-016-0157-8.
摘要
Abstract
In this study, a high-throughput screening method was established through the 24-square deep-well microliter plate (MTP) fermentation and micro-plate detection for large scale screening of the mutants. It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy ion beam irradiation. Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation. The filter paper activity (FPA) and β-Glucosidase (BGL) activity of ,Aspergillus niger, H11201 had increased 38.74% and 63.23% separately compared with ,Aspergillus niger, H11 by shaking flask fermentation and genetically stable after been passaged to nine generations. The results indicate that the high-throughput screening method can be used for the quick breeding of ,Aspergillus niger, with high cellulase activity.
关键词
Keywords
High-throughput screening methodAspergillus niger H11Cellulase12C6+ ion beam
references
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