Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator

Xiao-Qing Dong; Qing Lin; Jie Hu; Liang Huang; Kun Yue; Lu Wang; Jia-Fei Zhang; Mei-Ling Lu

doi:10.1007/s41365-018-0407-z

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Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator

NUCLEAR CHEMISTRY, RADIOCHEMISTRY, NUCLEAR MEDICINE | Updated：2021-02-07

- Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator
- Nuclear Science and Techniques Vol. 29, Issue 5, Article number: 67(2018)
- Affiliations：
  
  1.Department of Radiation Oncology, Tenth People’s Hospital affiliated to Tongji University, Shanghai 200072, China;
  2.Medical Equipment department, Tenth People’s Hospital affiliated to Tongji University, Shanghai, 200072, China;
  3.Department of Radiation Oncology, NO.9 People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 201999, China)
  4.Central Lab, Tenth People’s Hospital affiliated to Tongji University, Shanghai, 200072, China;
- Author bio：
  
  Corresponding author
- Funds：
- DOI：10.1007/s41365-018-0407-z
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Xiao-Qing Dong, Qing Lin, Jie Hu, et al. Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator. [J]. Nuclear Science and Techniques 29(5):67(2018)
DOI：

Xiao-Qing Dong, Qing Lin, Jie Hu, et al. Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator. [J]. Nuclear Science and Techniques 29(5):67(2018) DOI： 10.1007/s41365-018-0407-z.

摘要

Abstract

To establish and validate a method for cell irradiation in 96-well and 6-well plates using a linear accelerator, three irradiation methods (,G0B0F40, G0B1.5F40, and ,G180B1.5F40,) were designed to irradiate cell culture plasticware simulated with RW3 slab phantom and polystyrene. The difference between the actual physical measured dose and the preset dose was compared among the three methods under the preparatory conditions of 2, 4, 6, 8, and 10 Gy. MDA-MB-231 cells were analyzed by using a cell proliferation assay and a clonogenic assay to verify the difference between the three cell irradiation methods on cell radiosensitivity. For each preset dose, the difference between the actual measured dose and the preset dose was the lowest for ,Method G0B1.5F40, the second lowest for ,Method G180B1.5F40, and the maximum for ,Method G0B0F40,. The ranges of the differences were −0.28% to 0.02%, −2.17% to −1.80%, and −4.92% to −4.55%, and 0.31% to −0.12%, −3.42% to −2.86%, and −7.31% to −6.92%, respectively, for 96-well and 6-well plates. The cell culture experiments proved that ,Method G0B1.5F40, was an accurate, effective, simple, and practical irradiation method. The most accurate and effective cell irradiation method should always be used, as it will reduce dose differences and instability factors, and provide improved accuracy and comparability for laboratories researching cellular radiosensitivity.

关键词

Keywords

Linear acceleratorRadiotherapyCell irradiation methodsCell culture plateDose

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Related Institution

Advanced Energy Science and Technology Guangdong Laboratory

School of Nuclear Science and Technology, University of Chinese Academy of Sciences

Department of Radiation Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College

Institute of Modern Physics, Chinese Academy of Sciences

Institute of Health and Medical Technology, Hefei Institute of Materials Science, Chinese Academy of Sciences

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