Synchrotron infrared spectral regions as signatures for foodborne bacterial typing

Ya-Di Wang; Xue-Ling Li; Jun Hu; Jun-Hong Lü

doi:10.1007/s41365-019-0554-x

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Synchrotron infrared spectral regions as signatures for foodborne bacterial typing

NUCLEAR PHYSICS AND INTERDISCIPLINARY RESEARCH | Updated：2021-02-01

- Synchrotron infrared spectral regions as signatures for foodborne bacterial typing
- Nuclear Science and Techniques Vol. 30, Issue 2, Article number: 25(2019)
- Affiliations：
  
  1.Division of Physical Biology and CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences (CAS), Shanghai 201800, China.
  2.University of Chinese Academy of Sciences, Beijing 100049, China
  3.Shanghai Center for Bioinformation Technology, Shanghai Industrial Technology Institute, Shanghai 201203, China.
- Author bio：
  
  Xue-Ling Li. E-mail address: xlli@scbit.org
  Jun-Hong Lü. E-mail address: lujunhong@sinap.ac.cn
- Funds：
- DOI：10.1007/s41365-019-0554-x
  CLC：
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Ya-Di Wang, Xue-Ling Li, Jun Hu, et al. Synchrotron infrared spectral regions as signatures for foodborne bacterial typing. [J]. Nuclear Science and Techniques 30(2):25(2019)
DOI：

Ya-Di Wang, Xue-Ling Li, Jun Hu, et al. Synchrotron infrared spectral regions as signatures for foodborne bacterial typing. [J]. Nuclear Science and Techniques 30(2):25(2019) DOI： 10.1007/s41365-019-0554-x.

摘要

Abstract

Fourier transform infrared (FTIR) spectroscopy has emerged as a viable alternative to biochemical and molecular biology techniques for bacterial typing with advantages such as short analysis time, low cost, and laboratorial simplicity. In this study, synchrotron radiation-based FTIR (SR-FTIR) spectroscopy with higher spectral quality was successfully applied to 16 types foodborne pathogenic bacteria. Combined with principal component analysis (PCA) and hierarchical cluster analysis (HCA), we found that the specific spectral region is 1300-1000 cm,-1, which reflects the information of phosphate compounds and polysaccharides, and can be used as the signature region to cluster the strains into a similar groups with this genetic taxonomic method. These findings demonstrated that FTIR spectra combined with HCA have a great potential in quickly typing bacteria depending on their biochemical signatures.

关键词

Keywords

Synchrotron radiationFTIRSpectral signatureBacterial typingPCAHCA

references

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