Evaluation of sex specificity on oxidative stress induced in lungs of mice irradiated by ¹²C⁶⁺ ions

Evaluation of sex specificity on oxidative stress induced in lungs of mice irradiated by 12 C 6+ ions 1 first-author 1 corresp Corresponding author. E-mail address: zhangh@impcas.ac.cn Corresponding author. E-mail address: zhangh@impcas.ac.cn zhangh@impcas.ac.cn 2 Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Gansu Agricultural University, Lanzhou 730070, China Gansu Agricultural University, Lanzhou 730070, China The aim of this work is to identify if there is sex specificity on 12 C 6+ ion-induced oxidative damage in mouse lung at different time points. Kun-Ming mice were divided into two groups, each composed of six males and six females: control group and irradiation group with a single acute dose of 4 Gy. Animals were sacrificed at 2, 4 and 12 h respectively, there lungs were removed immediately, and the oxidative stress-related biomarkers were measured by Diagnostic Reagent Kits. The results showed that the relative activities of superoxide dismutase (4 h), catalase (2 h) and Se-dependent glutathione peroxidase (12 h) have significant changes ( P < 0.05) between male groups and female groups, suggesting that the lungs of male mice are more sensitive to counteracting the oxidative challenge. Moreover, higher levels of malondiadehyde and lower contents of glutathione were also found in males, indicating that oxidative stress induced by 12 C 6+ ion is pronounced in the lungs of males. We thought that these sex-responded differences may be attributed to the influence of sex hormones. Sex specificity Oxidative stress 12 C 6+ ion Mouse lung References Miyamoto T , Yamaaoto N , Nishimura H , et al . Radiother Oncol , 2003 , 66 : 127 - 140 . Radiother Oncol Zhang H , Zhang X , Yuan Z G , et al . J Radiat Res , 2006 , 47 : 131 - 134 . J Radiat Res Baldacchino G , Trupin-Wasselin V , Bouffard S , et al . Can J Physiol Phamacol , 2001 , 79 : 180 - 183 . Can J Physiol Phamacol Yamaguchi H , Uchihori Y , Yasuda N , et al . J Radiat Res , 2005 , 46 : 333 - 341 . J Radiat Res Meesungnoen J , Jay-Gerin J P . J Radiat Res , 2005 , 164 : 688 - 694 . J Radiat Res Hill P R . Br J Radiol , 2005 , 27 ( supplement ): 75 - 81 . Br J Radiol Asai K , Hiki N , Mimura Y , et al . Shock , 2001 , 6 : 340 - 343 . Shock Coto-Montes A , Boga J A , Tomas-Zapico C , et al . Free Radic Biol Med , 2001 , 30 : 785 - 792 . Free Radic Biol Med Silasi G , Diaz-Heijtz R , Besplug J , et al . Biochem Biophys Res Commun , 2004 , 325 : 1223 - 1235 . Biochem Biophys Res Commun Kovalchuk O , Burke P , Besplug J , et al . Muta Res , 2004 , 548 : 75 - 84 . Muta Res Pogribny I , Raiche J , Slovack M , et al . Biochem Biophys Res Commun , 2004 , 320 : 1253 - 1261 . Biochem Biophys Res Commun Zhang H , Xie Y , Zhou Q M , et al . Adv Space Res , 2006 , 38 : 1148 - 1151 . Adv Space Res Pathak P , Dey S K , Sarma A , et al . Mutat Res , 2007 , 632 : 58 - 68 . Mutat Res Valavanidis A , Vlahogianni T , Dassenakis M , et al . Ecotoxicol Environ Saf , 2006 , 64 : 178 - 189 . Ecotoxicol Environ Saf Houten B V , Woshner V , Santos J H , DNA Repair , 2006 , 5 : 145 - 152 . DNA Repair Shi X . J Inorg Biochem , 1994 , 56 : 155 - 165 . J Inorg Biochem Ansari M A , Joshi G , Huang Q . Free Radic Biol Med , 2006 , 41 : 1694 - 1703 . Free Radic Biol Med Bodel P , Dillard G M , Kaplan S S , et al . J Lab Clin Med , 1972 , 80 : 373 - 384 . J Lab Clin Med Behl C , Holsboer F . Trends Pharmacol Sci , 1999 , 20 : 441 - 444 . Trends Pharmacol Sci Borras C , Sastre J , Garcia-Sala D , et al . Free Radic Biol Med , 34 : 546 - 552 . Free Radic Biol Med Li C , Sang N , Wang Q . Ecotoxicol Environ Saf , 2006 , 65 : 134 - 139 . Ecotoxicol Environ Saf Pena-Llopis S , Ferrando M D , Pena J B . Aquat Toxicol , 2003 , 65 : 337 - 360 . Aquat Toxicol Habib G M , Shi Z Z , Liebeman M W . Free Radic Biol Med , 2007 , 42 : 191 - 201 . Free Radic Biol Med Vairetti M , Griffni P , Pietrocola G , et al . Free Radic Biol Med , 2001 , 31 : 954 - 961 . Free Radic Biol Med Shimizu T , Iwanaga M , Yasunaga A , et al . Mol Neurobiol , 1998 , 18 : 299 - 310 . Mol Neurobiol Lee J H , Lee Y M , Park J W . Biochem Biophys Res Commu , 2005 , 334 : 298 - 305 . Biochem Biophys Res Commu 10.1016/S1001-8042(08)60016-0 Q691.5 Supported by grants from the National Natural Science Foundation of China (10675151) , the Key Scientific Technology Research Projects of Gansu Province (2GS052-A43-008-02, 2GS063-A43-012, O702NKDA045) , the Scientific Technology Research Project of Lanzhou-Chinese Academy of Sciences (07-2-07) and the Program of Western Light (O760160XB0) 2007-10-16 2008-02-20 2021-01-19 2008 Nuclear Science and Techniques 01 19