The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line

Xiuli GAO; Shunfang YANG; Yongli YU; Meiping SHI; Lanxiang ZHAO; Jianding YE; Jianying LU

doi:10.1016/S1001-8042(09)60006-3

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The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE | Updated：2021-01-29

- The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line
- Nuclear Science and Techniques Vol. 19, Issue 5, (2008)
- Affiliations：
  
  1.Department of Nuclear Medicine, Shanghai No.6 Hospita Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China
  2.Department of Nuclear Medicine, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  3.Department of Pathology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  4.Department of Radiology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  5.Department of Prenatal Diagnosis, Maternal and Child Health Hospital Affiliated to Tongji University, Shanghai 200040, China
- Author bio：
  
  Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net
  Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net
- Funds：
- DOI：10.1016/S1001-8042(09)60006-3
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Cite this article
Xiuli GAO, Shunfang YANG, Yongli YU, et al. The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line. [J]. Nuclear Science and Techniques 19(5):290-296(2008)
DOI：

Xiuli GAO, Shunfang YANG, Yongli YU, et al. The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line. [J]. Nuclear Science and Techniques 19(5):290-296(2008) DOI： 10.1016/S1001-8042(09)60006-3.

摘要

Abstract

The study was to investigate the role of pinhole single photon emission computed tomography (SPECT), the human pulmonary adenocarcinoma bone-seeking metastasis cell line SPC-A-1BM was used. These cells form typical osteolytic bone metastases when inoculated into the arterial circulation of NIH-Beige-Nude-XD (BNX) mice ,via, the left ventricle. In order to evaluate the irradiation impact of ,99m,Tc-MDP versus X-ray on cells growth, we used six groups of SPC-A-1BM cells in our imaging scheme and irradiated by various doses of,99m,Tc-MDP (37, 74, 111, 370, 740 MBq) and X-ray(40 kV, 2 mA, 6 s) respectively. The cell's number of each group was well recorded in different exposure time (4, 8, 12, 24, 48, 72, 96 hours) . After that, SPC-A-1BM cells (1×106) were inoculated into the mice,via, left ventricle. We compared the results obtained with those different doses of ,99m,Tc-MDP using pinhole SPECT and conventional X-ray skeletal surveys. The data show that the cell-survival number of 111 MBq group has insignificant difference with that of X-ray and the dose is adequate to have an ideal image. Besides, it is important that the chromosome of the cells in the group of 111 MBq showed no irradiation-related damages in our test. These results implied that ,99m,Tc-MDP pinhole SPECT may provide another way other than conventional X-ray skeletal surveys in detecting bone metastasis of pulmonary adenocarcinoma in BNX mice.

关键词

Keywords

99mTc-MDPPinhole SPECTRadiationPulmonary adenocarcinomaBone metastasisAnimal model

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University of Chinese Academy of Sciences

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences

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