The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line

Xiuli GAO; Shunfang YANG; Yongli YU; Meiping SHI; Lanxiang ZHAO; Jianding YE; Jianying LU

doi:10.1016/S1001-8042(09)60006-3

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The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE | Updated：2021-01-29

- The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line
- Nuclear Science and Techniques
- Affiliations：
  
  1.Department of Nuclear Medicine, Shanghai No.6 Hospita Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China
  2.Department of Nuclear Medicine, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  3.Department of Pathology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  4.Department of Radiology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  5.Department of Prenatal Diagnosis, Maternal and Child Health Hospital Affiliated to Tongji University, Shanghai 200040, China
- Author bio：
  
  Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net
  
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  Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net
  
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- Funds：
- DOI：10.1016/S1001-8042(09)60006-3 CLC： R817.1
- Published：20 October 2008，
  
  Received：23 July 2008，
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Cite this article
Xiuli GAO, Shunfang YANG, Yongli YU, et al. The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line. [J]. Nuclear Science and Techniques 19(5):290-296(2008)
DOI：

Xiuli GAO, Shunfang YANG, Yongli YU, et al. The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line. [J]. Nuclear Science and Techniques 19(5):290-296(2008) DOI： 10.1016/S1001-8042(09)60006-3.

The pinhole SPECT for animal model of bone metastasis with SPC-A-1BM human pulmonary adenocarcinoma bone metastasis cell line 1 equal first-author 2 equal corresp Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net yuyongli56@hotmail.com 1 corresp Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net Corresponding authors. E-mail address: yuyongli56@hotmail.com, yzyg@sh163.net yuyongli56@hotmail.com 3 3 4 5 Department of Nuclear Medicine, Shanghai No.6 Hospita Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China Department of Nuclear Medicine, Shanghai No.6 Hospita Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China Department of Nuclear Medicine, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China Department of Nuclear Medicine, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China Department of Pathology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China Department of Pathology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China Department of Radiology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China Department of Radiology, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China Department of Prenatal Diagnosis, Maternal and Child Health Hospital Affiliated to Tongji University, Shanghai 200040, China Department of Prenatal Diagnosis, Maternal and Child Health Hospital Affiliated to Tongji University, Shanghai 200040, China The study was to investigate the role of pinhole single photon emission computed tomography (SPECT), the human pulmonary adenocarcinoma bone-seeking metastasis cell line SPC-A-1BM was used. These cells form typical osteolytic bone metastases when inoculated into the arterial circulation of NIH-Beige-Nude-XD (BNX) mice via the left ventricle. In order to evaluate the irradiation impact of 99m Tc-MDP versus X-ray on cells growth, we used six groups of SPC-A-1BM cells in our imaging scheme and irradiated by various doses of 99m Tc-MDP (37, 74, 111, 370, 740 MBq) and X-ray(40 kV, 2 mA, 6 s) respectively. The cell's number of each group was well recorded in different exposure time (4, 8, 12, 24, 48, 72, 96 hours) . After that, SPC-A-1BM cells (1×106) were inoculated into the mice via left ventricle. We compared the results obtained with those different doses of 99m Tc-MDP using pinhole SPECT and conventional X-ray skeletal surveys. The data show that the cell-survival number of 111 MBq group has insignificant difference with that of X-ray and the dose is adequate to have an ideal image. Besides, it is important that the chromosome of the cells in the group of 111 MBq showed no irradiation-related damages in our test. These results implied that 99m Tc-MDP pinhole SPECT may provide another way other than conventional X-ray skeletal surveys in detecting bone metastasis of pulmonary adenocarcinoma in BNX mice. 99m Tc-MDP Pinhole SPECT Radiation Pulmonary adenocarcinoma Bone metastasis Animal model References Husband J E S , Reznek R H . Imaging in Oncology . Oxford, U.K. : Isis Medical Media Ltd ., 1998 , 765 - 787 . Imaging in Oncology Blake G M , Park-Holohan S J , Cook G J , et al . Semin Nucl Med , 2001 , 31 : 28 - 49 . Semin Nucl Med Hamaoka T , Madewell J E , Podoloff D A , et al . J Clin Oncol , 2004 , 22 : 2942 - 2953 . J Clin Oncol Fogelman I . Eur J Nucl Med , 1982 , 7 : 506 - 509 . Eur J Nucl Med Arveschoug A K , Bertelsen H , Vammen B . Clin Nucl Med , 2002 , 27 : 249 - 254 . Clin Nucl Med Spanu A , Falchi A , Manca A , et al . J Nucl Med , 2004 , 45 : 40 - 48 . J Nucl Med Profanter C , Gabriel M , Wetscher G J , et al . Surg Today , 2004 , 34 : 493 - 497 . Surg Today Carlier T , Oudoux A , Mirallié E , et al . Eur J Med Mol Imaging , 2008 , 35 : 637 - 643 . Eur J Med Mol Imaging Jemal A , Travis W D , Tarone R E , et al . Int J Cancer , 2003 , 105 : 101 - 107 . Int J Cancer Rybak L D , Rosenthal D I . Q J Nucl Med , 2001 , 45 : 53 - 64 . Q J Nucl Med Schirrmeister H , Guhlnamm A , Kotzerke J , et al . J Clin Oncol , 1999 , 17 : 2381 - 2389 . J Clin Oncol Yoneda T , Williams P J , Hiraga T , et al . J Bone Miner Res , 2001 , 16 : 1486 - 1495 . J Bone Miner Res Kang Y , Siegel P M , Shu W , et al . Cancer Cell , 2003 , 3 : 537 - 549 . Cancer Cell Bacher K , Thierens H M . Nucl Med Commun , 2005 , 26 : 581 - 586 . Nucl Med Commun Huang L , Snyder A R , Morgan W F . Oncogene , 2003 , 22 : 5848 - 5854 . Oncogene Yang S F , Dong Q G , Yao M , et al . Cancer , 2006 , 26 : 1059 - 1063 (In Chinese) . Cancer Zietman A L , Sugiyama E , Ramsay J R , et al . Int J Cancer , 1991 , 47 : 755 - 759 . Int J Cancer O′Sullivan J M , Cook G J . Q J Nucl Med , 2002 , 46 : 152 - 159 . Q J Nucl Med Li J , Jaszczak R J , Greer K L , et al . Phys Med Biol , 1994 , 39 : 165 - 176 . Phys Med Biol Mitelman F . Mutat Res , 2000 , 462 : 247 - 253 . Mutat Res Lengauer C , Kinzler K W , Vogelstein B . Nature , 1998 , 396 : 643 - 649 . Nature Lorimore S A , Kadhim M A , Pocock D A , et al . Proc Natl Acad Sci U S A , 1998 , 95 : 5730 - 5733 . Proc Natl Acad Sci U S A Watson G E , Lorimore S A , Macdonald D A , et al . Cancer Res , 2000 , 60 : 5608 - 5611 . Cancer Res Lorimore S A , Coates P J , Scobie G E , et al . Oncogene , 2001 , 20 : 7085 - 7095 . Oncogene Malkinson A M . Cancer Res , 1992 , 52 ( 9 Suppl ): 2670s - 2676s . Cancer Res Sargent L M , Ensell M X , Ostvold A C , et al . Toxicol Appl Pharmacol , 2008, Feb 8 . [Epub ahead of print] . 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