Development of a soft X-ray microprobe for single cell radiobiology

Liang CHEN; Jingwen YAN; Shiping JIANG; Yang YU

doi:10.13538/j.1001-8042/nst.20.82-86

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Development of a soft X-ray microprobe for single cell radiobiology

SYNCHROTRON TECHNOLOGY AND APPLICATIONS | Updated：2021-01-29

- Development of a soft X-ray microprobe for single cell radiobiology
- Nuclear Science and Techniques Vol. 20, Issue 2, (2009)
- Affiliations：
  
  1.National Synchrotron Radiation Laboratory, University of Science & Technology of China, Hefei 230029, China
- Author bio：
  
  Corresponding author. E-mail address: spjiang@ustc.edu.cn
- Funds：
- DOI：10.13538/j.1001-8042/nst.20.82-86
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Liang CHEN, Jingwen YAN, Shiping JIANG, et al. Development of a soft X-ray microprobe for single cell radiobiology. [J]. Nuclear Science and Techniques 20(2):82-86(2009)
DOI：

Liang CHEN, Jingwen YAN, Shiping JIANG, et al. Development of a soft X-ray microprobe for single cell radiobiology. [J]. Nuclear Science and Techniques 20(2):82-86(2009) DOI： 10.13538/j.1001-8042/nst.20.82-86.

摘要

Abstract

An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells. The facility used synchrotron radiation as soft X-ray source. A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined doses. The diameter of microprobe at the target position was about 2 µm by scanning a knife-edge with an AXUV photo diode. The fluxes of soft X-rays at 516.7 eV (2.4 nm) were about 5.4×10,4, photons/s.100mA measured with the photo diode. The absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA. A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological investigations. The soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time.

关键词

Keywords

Synchrotron radiationSoft X-raysMicroprobeRadiation biology

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Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences

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