Directly radiolabeled phage with spleen-targeting specificity

Liyan SUN; Kun LIANG; Xiangyun WANG; Taiwei CHU

doi:10.13538/j.1001-8042/nst.22.217-223

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Directly radiolabeled phage with spleen-targeting specificity

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE | Updated：2021-01-29

- Directly radiolabeled phage with spleen-targeting specificity
- Nuclear Science and Techniques
- Affiliations：
  
  1.Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Author bio：
  
  Corresponding author. E-mail address: twchu@pku.edu.cn
  
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- DOI：10.13538/j.1001-8042/nst.22.217-223 CLC：
- Published：20 August 2011，
  
  Received：25 January 2011，
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Liyan SUN, Kun LIANG, Xiangyun WANG, et al. Directly radiolabeled phage with spleen-targeting specificity. [J]. Nuclear Science and Techniques 22(4):217-223(2011)
DOI：

Liyan SUN, Kun LIANG, Xiangyun WANG, et al. Directly radiolabeled phage with spleen-targeting specificity. [J]. Nuclear Science and Techniques 22(4):217-223(2011) DOI： 10.13538/j.1001-8042/nst.22.217-223.

Directly radiolabeled phage with spleen-targeting specificity 1 equal first-author 1 equal 1 1 corresp Corresponding author. E-mail address: twchu@pku.edu.cn Corresponding author. E-mail address: twchu@pku.edu.cn twchu@pku.edu.cn Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China Phage display technique is a powerful approach for discovering new tumor- and organ-targeting ligands, and radiolabeled phage has a potential to analyze the phage-binding sensitivity and specific imaging. In this study, phage II (the spleen-targeting phage) in mice was isolated after three rounds biopanning, and labeled by 99m Tc using mercaptoacetyltriglycine (MAG 3 ) as chelator to evaluate their binding properties in vivo . The amount of phage II eluted from spleen was enriched by plague assay each round. 99m Tc-MAG 3 -phage II showed the less retention in blood at any time point than half that of 99m Tc-MAG 3 -phage I (the radiolabeled original Ph.D-12 phage as control). The accumulation in spleen between 99m Tc-MAG 3 -phage I and II was of different tendency. The highest uptake of 99m Tc- MAG 3 -phage II in spleen was 24.80 %ID/g at 30 min; and of 99m Tc-MAG 3 -phage I, 30.93% ID/g at 5 min. After circulating 99m Tc-MAG 3 -phage II for 120 min, its accumulation in spleen decreased though higher than that of 99m Tc- MAG 3 -phage I. In other organs, the 99m Tc-MAG 3 -phage II showed low retention and high spleen-to-organ or tissue ratios. 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Acta 10.13538/j.1001-8042/nst.22.217-223 Supported by National Natural Science Foundation of China (No. 20771011 and 21071010) and the Ministry of Science and Technology of China (No. 2006CB705700) 2011-01-25 2011-08-20 2021-01-19 2011 Nuclear Science and Techniques 04 22

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