Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I

Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I 1 2 first-author 1 2 1 2 1 1 1 corresp Corresponding author. E-mail address: hujun@sinap.ac.cn Corresponding author. E-mail address: hujun@sinap.ac.cn hujun@sinap.ac.cn Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/ sensing technologies. In this paper, we report that the fluorescence of the double-stranded DNA (dsDNA) and SYBR Green I (SG) system (dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe 2 O 3 @DMSA) and gold nanoparticles in suitable concentrations, but positively charged nanoparticles quench the fluorescence. Effects of the Fe 2 O 3 @DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities. The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA. However, the dsDNA concentration determines the fluorescence amplitude. It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG. The finding may be useful in sensitive biomolecular detection. Surface charges Fluorescence enhancement Nanoparticles Double-stranded DNA SYBR Green I References Sai S , Ichikawa D , Tomita H , et al . Anticancer Res , 2007 , 27 : 2747 - 2751 . 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J Am Chem Soc 10.13538/j.1001-8042/nst.23.247-251 2012-01-20 2012-08-20 2021-01-19 2012 Nuclear Science and Techniques 04 23