A proteomics analysis for certain signature proteins of rabbit lacrimal passages after ¹²⁵I seeds brachytherapy

A proteomics analysis for certain signature proteins of rabbit lacrimal passages after 125 I seeds brachytherapy 1 first-author 1 1 2 1 1 corresp Corresponding author. E-mail address: qingjiema@yahoo.com.cn Corresponding author. E-mail address: qingjiema@yahoo.com.cn qingjiema@yahoo.com.cn China-Japan Union Hospital of Jilin University, Changchun 130033, China China-Japan Union Hospital of Jilin University, Changchun 130033, China Hong Qi Hospital of Mudanjiang Medical College, Mudanjiang 157011, China Hong Qi Hospital of Mudanjiang Medical College, Mudanjiang 157011, China To search for certain signature proteins and the expression profiles in lacrimal passage stenosis, rabbit models of lacrimal passage stenosis were treated by 125 I seed brachytherapy. All the signature proteins were separated by two-dimensional electrophoresis, and identified by mass spectrometry. The results show that the up-regulated proteins are peptidyl-prolyl cis-trans isomerase A (PPIase A), and epidermal fatty acid-binding protein (E-FABP), while the down-regulated proteins are myosin light chain 1(isomer of skeletal muscle), myosin light polypeptide 6 (isomer 1 of smooth muscle and non-muscle), myosin light chain 1(isomer of slow-twitch muscle A), isomer 2 of ERC protein 2, and α-crystallin family protein. The proteins may play a role in healing the wound and regulating synaptic active zone of neurons due to correlation to cell apoptosis, proliferation and migration of smooth muscle cell. These provide molecular mechanism for preventing stenosis and restenosis of lacrimal passage. Lacrimal passage stenosis Radioactive nuclide Probing of lacrimal passage Differential expression protein Proteome References Zhang X C . Intern J Ophthalmol , 2004 , 4 : 746 - 748 . Intern J Ophthalmol Jin L Y , Zhao Z , Ma Q J . Chin J Gerontology , 2005 , 5 : 565 - 566 . Chin J Gerontology Robb R M . Ophthalmol , 1998 , 105 : 1307 - 1309 . Ophthalmol Gao S , Ma Q J , Cui Q . Chin J Nucl Med , 2006 , 26 : 378 - 380 . Chin J Nucl Med Tannu N S , Hemby S E . Nat Protoc , 2006 , 1 : 1732 - 1742 . Nat Protoc Klein M J , Siegal G P , Am J Clin Pathol , 2006 , 125 : 555 - 581 . Am J Clin Pathol Kern G , Kern D , Schmid F X . FEBS Lett , 1994 , 348 : 145 - 148 . FEBS Lett Capano M , Virji S , Crompton M . Biochem J , 2002 , 363 : 29 - 36 . Biochem J Leverson J D , Ness S A . Mol Cell , 1998 , 1 : 203 - 211 . Mol Cell Fareh J , Martel R , Kermani P . Circulation , 1999 , 99 : 1477 - 1484 . Circulation Sobue K , Hayashi K , Nishida W . Mol Cell Biochem , 1999 , 190 : 105 - 118 . Mol Cell Biochem Ohtsuka T , Takao-Rikitsu E , Inoue E . J Cell Biol , 2002 , 158 : 577 - 590 . J Cell Biol Wang Y , Liu X , Biederer T . Proc Nat Acad Sci USA , 2002 , 99 : 14464 - 14469 . Proc Nat Acad Sci USA Monier S , Jollivet F , Janoueix-Lerosey I . Traffic , 2002 , 3 : 289 - 297 . Traffic Nakata T , Kitamura Y , Shimizu K . Gene Chromosome Cancer , 1999 , 25 : 97 - 103 . Gene Chromosome Cancer Wang Y , Okamoto M , Schmitz F . Nature , 1997 , 388 : 593 - 598 . Nature Wang Y , Sugita S , Sudhof T C . J Biol Chem , 2002 , 275 : 20033 - 20044 . J Biol Chem Jaewon K , Moonseok N , Seho K . J Biol Chem , 2003 , 278 : 42377 - 42385 . J Biol Chem Kusakari Y , Ogawa E , Owada Y . Mol Cell Biochem , 2006 , 284 : 183 - 188 . Mol Cell Biochem Chow C W , Herrera Abreu M T , Suzuki T . Am J Respir CellMol , 2003 , 29 : 427 - 431 . Am J Respir CellMol Bennaars-Eiden A , Higgins L , Hertzel A V . J Biol Chem , 2002 , 27 : 50693 - 50702 . J Biol Chem Sehroeter M , Jander S , Witte O W . Neurosci , 1999 , 59 : 1367 - 1377 . Neurosci Jochen G , Yoh M , Georg W K . Brain Res Brain Rev , 1995 , 20 : 269 - 287 . Brain Res Brain Rev Zielasek J , Hartung H P . Neuroimmun , 1991 , 6 : 191 - 222 . Neuroimmun Wiessner C , Brink I , Lorenz P . Neurosci , 1996 , 72 : 947 - 958 . Neurosci Stoll G , Jander S , Sehroeter M . Prog Neurobiol , 1998 , 56 : 149 - 171 . Prog Neurobiol Rupalatha M , P V R . Experiment Cell Res , 2005 , 306 : 203 - 215 . Experiment Cell Res 10.13538/j.1001-8042/nst.21.227-232 Supported by Nature Science Foundation of Jilin Province (No.20075323) ; Project of Science and Technology of Changchun City (2007GH25) ; 2010-05-27 2010-08-20 2021-01-19 2010 Nuclear Science and Techniques 04 21