Pigment analysis of a color-leaf mutant in Wandering Jew (Tradescantia fluminensis) irradiated by carbon ions

Pigment analysis of a color-leaf mutant in Wandering Jew ( Tradescantia fluminensis ) irradiated by carbon ions 1 2 first-author 1 1 1 corresp Corresponding author. E-mail address: wjli@impcas.ac.cn Corresponding author. E-mail address: wjli@impcas.ac.cn wjli@impcas.ac.cn Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Graduate University of Chinese Academy of Sciences, Beijing 100049, China Graduate University of Chinese Academy of Sciences, Beijing 100049, China Many mutants of plant induced by heavy ion beam irradiation have been reported in recent years, but leaf anthocyan mutants induced by ion irradiation in evergreen were rarely found. In this study, a color-leaf mutant with purple leaves, stems and petals was isolated from clones of Wandering Jew irradiated by 95.8 MeV/u carbon ion beam. The concentration and histological distribution of leaf pigment were surveyed in wild type and mutant. In mutant, contents of total chlorophylls (Chl), chlorophyll a (Chl a ), chlorophyll b (Chl b ) and carotenoids (Car) decreased significantly, while concentration of the anthocyanins was 6.2-fold higher than that of wild type. Further composition analysis of anthocyanins by electrospray ionization mass spectrometry (ESI-MS) indicated that the purple pigmentation of leaves in mutant was caused by accumulation of petunidin anthocyanin. Microscopic examination showed that most petunidin anthocyanin accumulated in the lower epidermis, and little in vascular parenchyma of mutant, while there was no pigment in wild type. Meanwhile, in spongy parenchyma of mutant we observed little Chl, which the wild type abounds in. In conclusion, the color-leaf mutant of Wandering Jew induced by irradiation of carbon ions was improved in ornamental value, and it could be contribute to variation in level, component and distribution of foliar pigment. The possible mutation mechanisms were discussed. Foliar pigment Wandering Jew Carbon ion beam Irradiation References Goodhead D T . Radiat Environ Biophys , 1995 , 34 : 67 - 72 . Radiat Environ Biophys Okamura M , Yasuno N , Ohtsuka M , et al . Nucl Instrum Meth Phys Res B , 2003 , 206 : 574 - 578 . Nucl Instrum Meth Phys Res B Yamaguchi H , Nagatomi S , Morishita T , et al . Nucl Instrum Meth Phys Res B , 2003 , 206 : 561 - 564 . Nucl Instrum Meth Phys Res B Sasaki K , Aida R , Niki T , et al . Plant Biotechnol , 2008 , 25 : 81 - 89 . Plant Biotechnol Matsumura A , Nomizu T , Furutani N , et al . Sci Hortic , 2009 , 123 : 558 - 561 . Sci Hortic Miyazaki K , Suzuki K , Iwaki K , et al . Plant Biotechnol , 2006 , 23 : 163 - 167 . Plant Biotechnol Kondo E , Nakayama M , Kameari N , et al . Plant Biotechnol , 2009 , 26 : 565 - 569 . Plant Biotechnol Wu D , Hou S , Qian P , et al . Sci. Hortic , 2009 , 121 : 462 - 467 . Sci. Hortic Ahmad Z , Hassan A , Idris N , et al . J Nucl Relat Technol , 2006 , 3 : 1 - 8 . J Nucl Relat Technol Ciuffo M , Masenga V , Turina M . Arch Virol , 2006 , 151 : 1235 - 1241 . Arch Virol Marciulioniene D , Montvydiene D , Kiponas D , et al . Environ Toxicol , 2004 , 19 : 346 - 350 . Environ Toxicol Lichtenthaler H , Wellburn A . Biochem Soc Trans , 1983 , 11 : 591 - 592 . Biochem Soc Trans Xie D , Sharma S , Wright E , et al . Plant J , 2006 , 45 : 895 - 907 . Plant J Gould K , Markham K , Smith R , et al . J Exp Bot , 2000 , 51 : 1107 - 1115 . J Exp Bot Wu X , Prior R . J. Agric. Food Chem , 2005 , 53 : 2589 - 2599 . J. Agric. Food Chem Abe T , Matsuyama T , Sekido S , et al . J Radiat Res , 2002 , 43 : 157 - 161 . J Radiat Res Shikazono N , Yokota Y , Kitamura S , et al . Genetics , 2003 , 163 : 1449 - 1455 . Genetics Bae C , Abe T , Nagata N , et al . Plant Sci , 2000 , 151 : 93 - 101 . Plant Sci Yu F , Fu A , Aluru M , et al . Plant Cell Environ , 2007 , 30 : 350 - 365 . Plant Cell Environ Manetas Y . Flora , 2006 , 201 : 163 - 177 . Flora Albert N , Lewis D , Zhang H , et al . J Exp Bot , 2009 , 60 : 2191 - 2202 . J Exp Bot Phanchaisri B , Chandet R , Yu L D , et al . Surf Coat Technol , 2007 , 201 : 8024 - 8028 . Surf Coat Technol Oren-Shamir M . Plant Sci , 2009 , 177 : 310 - 316 . Plant Sci Zhou L B , Li W J , Yu L X , et al . Int J Radiat Biol , 2006 , 82 : 473 - 481 . Int J Radiat Biol 10.13538/j.1001-8042/nst.22.77-83 Supported by the superintendents' foundation of Institute of Modern Physics, Chinese Academy of Sciences (0906020SZO) . 2010-12-05 2011-04-20 2021-01-19 2011 Nuclear Science and Techniques 02 22