Effect of p53 on lung carcinoma cells irradiated by carbon ions or X-rays

Effect of p53 on lung carcinoma cells irradiated by carbon ions or X-rays 1 first-author 1 corresp Corresponding author. E-mail address: zhangh@impcas.ac.cn Corresponding author. E-mail address: zhangh@impcas.ac.cn zhangh@impcas.ac.cn 1 1 1 1 2 Department of Medical Radiation Biology, Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China Department of Medical Radiation Biology, Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China Department of Radiotherapy, Institute of Medicine Science in Gansu Province, Lanzhou 730000, China Department of Radiotherapy, Institute of Medicine Science in Gansu Province, Lanzhou 730000, China The study is to investigate the feasibility and advantages of heavy ion beams on radiotherapy. The cellular cycle and apoptosis, cell reproductive death and p53 expression evaluated with flow cytometry, clonogenic survival assays and Western blot analysis were examined in lung carcinoma cells after exposure to 89.63 MeV/u carbon ion and 6 MV X-ray irradiations, respectively. The results showed that the number colonyforming assay of A549 was higher than that of H1299 cells in two radiation groups; A549 cellular cycle was arrested in G 2 /M in 12 h and the percentage of apoptosis ascended at each time point of carbon ion radiation with doses, the expression of p53 upregulated with doses exposed to X-ray or carbon ion. The cell number in G 2 /M of H1299 and apoptosis were increasing at all time points with doses in 12 C 6+ ion irradiation group. The results suggested that the effects of carbon ions or X rays irradiation on lung carcinoma cells were different, 12 C 6+ ion irradiation could have more effect on upregulating the expression of p53 than X-ray, and the upregulated expression of p53 might produce the cellular cycle G 2 /M arrested, apoptosis increasing; and p53 gene might affect the lung cancer cells radiosensitivity. 12 C 6+ ion irradiation Expression of p53 Cellular cycle and apoptosis Survival of cells X-ray References Dikomey E , Borgmann K , Brammer I et al . Toxicology , 2003 , 193 ( 1-2 ): 125 - 135 . Toxicology Distel LV , Neubauer S , Keller U et al . Radiother Oncol , 2006 , 81 ( 3 ): 257 - 263 . Radiother Oncol Peacock J H , Edwards H A , Mcmillan T R et al . Int J Radiat Biol , 1989 , 56 ( 5 ): 543 - 547 . Int J Radiat Biol Komarova E A , Chernov M V , Franks R , et al . EMBO J , 1997 , 16 ( 6 ): 1391 - 1400 . EMBO J Frenkel J , Sherman D , Fein A , et al . Oncogene , 1999 , 18 ( 18 ): 2901 - 2907 . Oncogene Komarova E A , Gudkov A V . Biochemistry (Mosc) , 2000 , 65 ( 1 ): 41 - 48 . Biochemistry (Mosc) Vanparys C , Maras M , Lenjou M , et al . Coen Toxicol in Vitro , 2006 , 20 ( 7 ): 1238 - 1248 . Coen Toxicol in Vitro Farmer G , Bargonetti J , Zhu H , et al . Nature , 1992 , 358 ( 6381 ): 83 - 86 . Nature El-Deiry W S , Tokino T , Velculescu V E , et al . Cell , 1993 , 75 ( 4 ): 817 - 825 . Cell Harper J W , Adami G R , Wei N , et al . Cell , 1993 , 75 ( 4 ): 805 - 816 . Cell Xiong Y , Hannon GJ , Zhang H , et al . Nature , 1993 , 366 ( 6456 ): 701 - 704 . Nature Vousden K H . Cell , 2000 , 103 ( 5 ): 691 - 694 . Cell Vogelstein B , Lane D , Levine A J . Nature , 2000 , 408 ( 6810 ): 307 - 310 . Nature Deng Y , Wu X . Proc Natl Acad Sci U. S. A , 2000 , 97 ( 22 ): 12050 - 12055 . Proc Natl Acad Sci U. S. A Brugarolas J , Chandrasekaran C , Gordon J I , et al . Nature , 1995 , 377 ( 6549 ): 552 - 557 . Nature Harris M P , Sutjipto S , Wills K N , et al . Cancer Gene Ther , 1996 , 3 ( 2 ): 121 - 130 . Cancer Gene Ther Nielsen L L , Maneval D C . Cancer Gene Ther , 1998 , 5 ( 1 ): 52 - 63 . Cancer Gene Ther Colletier P J , Ashoori F , Cowen D , et al . Int J Radiat Oncol Biol Phys , 2000 , 48 ( 5 ): 1507 - 1512 . Int J Radiat Oncol Biol Phys Koizumi H , Taguchi M , Kobayashi Y , et al . Instrum Method Phys Res, Sect B , 2003 , 208 ( 43 ): 161 - 165 . Instrum Method Phys Res, Sect B 10.13538/j.1001-8042/nst.20.146-151 Supported by the National Natural Science Foundation of China (10675151 and 10835011) , the Key Scientific Technology Research Projects of Gansu Province (0702NKDA045 and 0801NKDA001) and Science and technology brainstorm project of Lanzhou (07-2-07) 2009-01-20 2009-06-20 2021-01-19 2009 Nuclear Science and Techniques 03 20