Development of a soft X-ray microprobe for single cell radiobiology

Development of a soft X-ray microprobe for single cell radiobiology 1 first-author 1 1 corresp Corresponding author. E-mail address: spjiang@ustc.edu.cn Corresponding author. E-mail address: spjiang@ustc.edu.cn spjiang@ustc.edu.cn 1 National Synchrotron Radiation Laboratory, University of Science & Technology of China, Hefei 230029, China National Synchrotron Radiation Laboratory, University of Science & Technology of China, Hefei 230029, China An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells. The facility used synchrotron radiation as soft X-ray source. A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined doses. The diameter of microprobe at the target position was about 2 µm by scanning a knife-edge with an AXUV photo diode. The fluxes of soft X-rays at 516.7 eV (2.4 nm) were about 5.4×10 4 photons/s.100mA measured with the photo diode. The absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA. A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological investigations. The soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time. Synchrotron radiation Soft X-rays Microprobe Radiation biology References Folkard M , Vojnovic B , Prise K M , et al . Phys Med Biol , 1987 , 32 : 1615 - 1626 . Phys Med Biol Hill M A , Vecchia M D , Townsend K M S , et al . Phys Med Biol , 1998 , 43 : 351 - 363 . Phys Med Biol De Lara C M , Hill M A , Jenner T J , et al . Radiat Res , 2001 , 155 : 440 - 448 . Radiat Res Gobert F N , Lamoureux M , Herve du Penhoat M A , et al . Int J Radiat Biol , 2004 , 80 : 135 - 145 . Int J Radiat Biol Hill M A , Stevens D L , Bance D A , et al . Int J Radiat Biol , 2002 , 78 : 967 - 979 . Int J Radiat Biol Folkard M , Schettino G , Vojnovic B , et al . SPIE , 2001 , 4499 : 10 - 18 . SPIE Kobayashi K , Hieda, K , Maezawa H , et al . Int J Radiat Biol , 1991 , 59 : 643 - 650 . Int J Radiat Biol Prise K M , Folkard M , Michael B D . Oncogene , 2003 , 22 : 7043 - 7049 . Oncogene Schettino G , Folkand M , Michael B D , et al . Radiat Res , 2005 , 163 : 332 - 336 . Radiat Res Herve du Penhoat M A , Fayard B , Abel F , et al . Radiat Res , 1999 , 151 : 649 - 658 . Radiat Res Fayard B , Touati A , Abel F , et al . Radiat Res , 2002 , 157 : 128 - 140 . Radiat Res Jacobsen C . Trends in cell Biol , 1999 , 9 : 44 - 47 . Trends in cell Biol Cazaux J . J Microscopy , 1997 , 188 : 106 - 124 . J Microscopy Spector S J , Jacobsen C J , Tennant D M . J Vacuum Sci Tech , 1997 , 15 : 2872 - 2876 . J Vacuum Sci Tech Masini A , Batani D , Previdi F , et al . SPIE , 1998 , 3157 : 10 - 18 . SPIE Thompsom A , Lindau I , Attwood D , et al . X-ray data booklet , Lawrence Berkeley National Laboratory , 2001 , 5.18 - 5.24 . X-ray data booklet Fujisaki H , Takahashi S , Ohzeki H , et al . J Microscopy , 1996 , 182 : 79 - 83 . J Microscopy 10.13538/j.1001-8042/nst.20.82-86 2008-10-30 2009-04-20 2021-01-19 2009 Nuclear Science and Techniques 02 20