Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation

Ning LI; Hong ZHANG; Yanling WANG; Xiaohu WANG; Jifang HAO

doi:10.13538/j.1001-8042/nst.20.87-92

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Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation

LOW ENERGY ACCELERATOR, RAY TECHNOLOGY AND APPLICATIONS | Updated：2021-01-29

- Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation
- Nuclear Science and Techniques Vol. 20, Issue 2, (2009)
- Affiliations：
  
  1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  2.Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China
  3.Graduate School of Chinese Academy of Sciences, Beijing 100039, China
  4.Department of Radiotherapy, Gansu Tumor Hospital, Lanzhou 730050, China
- Author bio：
  
  Corresponding author. E-mail address: zhangh@impcas.ac.cn
  
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- Funds：
- DOI：10.13538/j.1001-8042/nst.20.87-92 CLC：
- Published：20 April 2009，
  
  Received：15 December 2008，
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Cite this article
Ning LI, Hong ZHANG, Yanling WANG, et al. Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation. [J]. Nuclear Science and Techniques 20(2):87-92(2009)
DOI：

Ning LI, Hong ZHANG, Yanling WANG, et al. Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation. [J]. Nuclear Science and Techniques 20(2):87-92(2009) DOI： 10.13538/j.1001-8042/nst.20.87-92.

Potential role of DNA-dependent protein kinase in cellular resistance to ionizing radiation 1 2 3 first-author 1 2 corresp Corresponding author. E-mail address: zhangh@impcas.ac.cn Corresponding author. E-mail address: zhangh@impcas.ac.cn zhangh@impcas.ac.cn 1 2 3 4 1 2 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China Graduate School of Chinese Academy of Sciences, Beijing 100039, China Graduate School of Chinese Academy of Sciences, Beijing 100039, China Department of Radiotherapy, Gansu Tumor Hospital, Lanzhou 730050, China Department of Radiotherapy, Gansu Tumor Hospital, Lanzhou 730050, China In this paper, we study the ability of DNA-PK-deficient (M059J) and -proficient (M059K) cells to undergo the rate of cellular proliferation, cell cycle distribution and apoptosis after 10 Gy X-ray irradiation, and the role of DNA-PK in radiosensitivity. The results showed that M059J cells exhibited hyper-radiosensitivity compared with M059K cells. A strong G2 phase arrest was observed in M059J cells post irradiation. Significant accumulation in the G2 phase in M059J cells was accompanied by apoptosis at 12 h. Altogether, the data suggested that DNA-PK may have two roles in mammalian cells after DNA damage, a role in DNA DSB repair and a second role in DNA-damaged cells to traverse a G2 checkpoint, by which DNA-PK may affect cellular sensitivity to ionizing radiation. DNA-dependent protein kinase (DNA-PK) Cell cycle checkpoint Apoptosis Radiosensitivity References Burma S , Chen D J . DNA Repair (Amst) , 2004 , 3 : 909 - 918 . DNA Repair (Amst) Jeggo P A . Radiat Res , 1998 , 150 : S80 - S91 . Radiat Res DiBiase S J , Zeng Z C , Chen R , et al . Cancer Res , 2000 , 60 : 1245 - 1253 . Cancer Res Iliakis G , Wang H , Perrault AR , et al . Cytogenet Genome Res , 2004 , 104 : 14 - 20 . Cytogenet Genome Res Smith G C , Jackson S P . Genes Dev , 1999 , 13 : 916 - 934 . Genes Dev Shiloh Y . Nat Rev Cancer , 2003 , 3 : 155 - 168 . Nat Rev Cancer Chan D W , Chen B P , Prithivirajsingh S , et al . Genes Dev , 2002 , 16 : 2333 - 2338 . Genes Dev Ding Q , Reddy Y V , Wang W , et al . Mol Cell Biol , 2003 , 23 : 5836 - 5848 . Mol Cell Biol Woo R A , Jack M T , Xu Y , et al . EMBO J , 2002 , 21 : 3000 - 3008 . EMBO J Wang S , Guo M , Ouyang H , et al . Proc Natl Acad Sci USA , 2000 , 97 : 1584 - 1588 . Proc Natl Acad Sci USA Candéias S M , Durum SK , Muegge K . Biochimie , 1997 , 79 : 607 - 612 . Biochimie Gurley K E , Vo K , Kemp C J . Cancer Res , 1998 , 58 : 3111 - 3115 . Cancer Res Danial N N , Korsmeyer S J . Cell , 2004 , 116 : 205 - 219 . Cell Strasser A , O'Connor L , Dixit V M . Annu Rev Biochem , 2000 , 69 : 217 - 245 . Annu Rev Biochem Hanahan D , Weinberg R A . Cell , 2000 , 100 : 57 - 70 . Cell Jäättelä M . Oncogene , 2004 , 23 : 2746 - 2756 . Oncogene Cryns V , Yuan J . Genes Dev , 1998 , 12 : 1551 - 1570 . Genes Dev Los M , Wesselborg S , Schulze-Osthoff K . Immunity , 1999 , 10 : 629 - 639 . Immunity Allalunis-Turner M J , Barron G M , 3rd Day R S , et al . Radiat Res , 1993 , 134 : 349 - 354 . Radiat Res Hosoi Y , Miyachi H , Matsumoto Y , et al . Int J Cancer , 1998 , 78 : 642 - 647 . Int J Cancer Solly K , Wang X , Xu X , et al . Assay Drug Dev Technol , 2004 , 2 : 363 - 372 . Assay Drug Dev Technol Decker P , Muller S . Curr Pharm Biotechnol , 2002 , 3 : 275 - 283 . Curr Pharm Biotechnol Soldani C , Scovassi A I . Apoptosis , 2002 , 7 : 321 - 328 . Apoptosis Duriez P J , Shah G M . Biochem Cell Biol , 1997 , 75 : 337 - 349 . Biochem Cell Biol Allalunis-Turner M J , Zia P K , Barron G M , et al . Radiat Res , 1995 , 144 : 288 - 293 . Radiat Res Lee S E , Mitchell R A , Cheng A , et al . Mol Cell Biol , 1997 , 17 : 1425 - 1433 . Mol Cell Biol Nilsson A , Sirzén F , Lewensohn R , et al . Cell Prolif , 1999 , 32 : 239 - 248 . Cell Prolif Holgersson A , Jernberg A R , Persson L M , et al . Int J Radiat Biol , 2003 , 79 : 611 - 621 . Int J Radiat Biol Holgersson A , Heiden T , Castro J , et al . Int J Radiat Oncol Biol Phys , 2005 , 61 : 915 - 921 . Int J Radiat Oncol Biol Phys Takata M , Sasaki M S , Sonoda E , et al . EMBO J , 1998 , 17 : 5497 - 5508 . EMBO J Stenerlöw B , Karlsson K H , Cooper B , et al . Radiat Res , 2003 , 159 : 502 - 510 . Radiat Res Yoshida M , Hosoi Y , Miyachi H , et al . Int J Radiat Biol , 2002 , 78 : 503 - 512 . Int J Radiat Biol 10.13538/j.1001-8042/nst.20.87-92 Supported by National Natural Science Foundation of China (10675151) and Western Talent Program of Chinese Academy of Sciences (0860260XBO) . 2008-12-15 2009-04-20 2021-01-19 2009 Nuclear Science and Techniques 02 20

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