Screening tumor-targeting bacteriophage particles by pre-clearing phage display

Kun LIANG; Yao LI; Taiwei CHU

doi:10.13538/j.1001-8042/nst.23.34-39

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Screening tumor-targeting bacteriophage particles by pre-clearing phage display

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE | Updated：2021-01-29

- Screening tumor-targeting bacteriophage particles by pre-clearing phage display
- Nuclear Science and Techniques Vol. 23, Issue 1, (2012)
- Affiliations：
  
  1.Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
- Author bio：
  
  Corresponding author. E-mail address: twchu@pku.edu.cn
  
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- DOI：10.13538/j.1001-8042/nst.23.34-39 CLC：
- Published：20 February 2012，
  
  Received：10 October 2011，
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Cite this article
Kun LIANG, Yao LI, Taiwei CHU. Screening tumor-targeting bacteriophage particles by pre-clearing phage display. [J]. Nuclear Science and Techniques 23(1):34-39(2012)
DOI：

Kun LIANG, Yao LI, Taiwei CHU. Screening tumor-targeting bacteriophage particles by pre-clearing phage display. [J]. Nuclear Science and Techniques 23(1):34-39(2012) DOI： 10.13538/j.1001-8042/nst.23.34-39.

Screening tumor-targeting bacteriophage particles by pre-clearing phage display 1 first-author 1 1 corresp Corresponding author. E-mail address: twchu@pku.edu.cn Corresponding author. E-mail address: twchu@pku.edu.cn twchu@pku.edu.cn Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China Beijing National Laboratory for Molecular Sciences, Radiochemistry and Radiation Chemistry Key Laboratory of Fundamental Science, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China Phage display technique provides a powerful approach for the discovery of new tumor-specific peptides. However, the peptides isolated through this technique usually did not possess high tumor-specific property. A pre-clearing step was introduced to increase the efficiency of biopanning by removal of particles that could interact with ubiquitously expressed cellular receptors in the non-target organs. The randomized Ph.D-CX 7 C phage library (Phage III) was first pre-cleared in normal mice to reduce vasculature- or organ-targeting phages to get the pre-cleared phage library, and then the tumor-targeting bacteriophage particles (Phage I) were screened from pre-clearing phage library in S180 tumor-bearing mice. The biodistribution results of 99m Tc-labeled phages in mice bearing S180 tumor show that the uptake of 99m Tc-labeled Phage I in tumor is high but low in normal organs, and the tumor-to-liver and tumor-to-spleen ratios of 99m Tc-labeled Phage I are higher than those of 99m Tc-labeled Phage II (tumor-specific phages screened from the original CX7C library) and Phage III (unscreened phages from the original CX7C library). It indicates that the yield of tumor-targeting bacteriophage particles could be improved and the non-specific binding in organs becomes weak. Consequently, the pre-clearing phage display method could improve the yield of positive hits by reducing the non-target organ accumulation of bacteriophage particles. In vivo phage display Pre-clearing phage library Tumor-targeting Radio-labeling References Spear M A , Breakefield X O , Beltzer J , et al . Cancer Gene Ther , 2001 , 8 : 506 - 511 . Cancer Gene Ther Liu M , Li C , Pazgier M , et al . Proc Natl Acad Sci USA , 2010 , 107 : 14321 - 14326 . Proc Natl Acad Sci USA Lee T Y , Wu H C , Tseng Y L , et al . Cancer Res , 2004 , 64 : 8002 - 8008 . Cancer Res Nicklin S A , White S J , Watkins S J , et al . Circulation , 2000 , 102 : 231 - 237 . Circulation Landon L A , Deutscher S L . J Cell Biochem , 2003 , 90 : 509 - 517 . J Cell Biochem Newton J R , Kelly K A , Mahmood U , et al . Neoplasia , 2006 , 8 : 772 - 780 . Neoplasia Arap W , Pasqualini R , Ruoslahti E . Science , 1998 , 279 : 377 - 380 . Science Pasqualini R , Ruoslahti E . Nature , 1996 , 380 : 364 - 366 . Nature Smith G P . Smith Lab Homepage . www.biosci.missouri.edu/smithGP/ , 2006 . Smith Lab Homepage Sun L Y , Liang K , Wang X Y , et al . Nucl Sci Tech , 2011 , 22 : 217 - 223 . Nucl Sci Tech Rusckowski M , Gupta S , Liu G Z , et al . J Nucl Med , 2004 , 45 : 1201 - 1208 . J Nucl Med Zou J , Dickerson M T , Owen N K , et al . Mol Biol Rep , 2004 , 31 : 121 - 129 . Mol Biol Rep Fan X M , Venegas R , Fey R , et al . Pharm Res , 2007 , 24 : 868 - 879 . Pharm Res Smith G P . Science , 1985 , 228 : 1315 - 1317 . Science Rajotte D , Arap W , Hagedorn M , et al . J Clin Invest , 1998 , 102 : 430 - 437 . J Clin Invest Joyce J A , Laakkonen P , Bernasconi M , et al . Cancer Cell , 2003 , 4 : 393 - 403 . Cancer Cell Kolonin M G , Sun J , Do K A , et al . FASEB J , 2006 , 20 : 979 - 981 . FASEB J Arap M A , Lahdenranta J , Mintz P J , et al . Cancer Cell , 2004 , 6 : 275 - 284 . Cancer Cell Sato M , Arap W , Pasqualini R . Oncology , 2007 , 21 : 1346 - 1352 . Oncology Ozawa M G , Zurita A J , Dias-Neto E , et al . Trends Cardiovasc Med , 2008 , 18 : 126 - 132 . Trends Cardiovasc Med Cardo-Vila M , Zurita A J , Giordano R J , et al . PLoS ONE , 2008 , 3 : e3452 . PLoS ONE Staquicini F I , Tandle A , Libutti S K , et al . Cancer Res , 2008 , 68 : 8419 - 8428 . Cancer Res Sun L Y , Chu T W , Wang Y , et al . Chem J Chin Univ , 2007 , 28 : 621 - 626 . Chem J Chin Univ Hardy B , Raiter A , Weiss C , et al . Peptides , 2007 , 28 : 691 - 701 . Peptides 10.13538/j.1001-8042/nst.23.34-39 2011-10-10 2012-02-20 2021-01-19 2012 Nuclear Science and Techniques 01 23

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