Total DNA of Glycyrrhiza uralensis transformed into Hansenula anomala by ion implantation: Preparing Glycyrrhizic acid in recombined yeasts

Total DNA of Glycyrrhiza uralensis transformed into Hansenula anomala by ion implantation: Preparing Glycyrrhizic acid in recombined yeasts 1 first-author 1 3 2 1 corresp Corresponding author. E-mail address: phmao@china.com Corresponding author. E-mail address: phmao@china.com phmao@china.com Center of Ion Beam Biotechnology, College of Physics and Technology, Xinjiang University, Urumqi 830008, China Center of Ion Beam Biotechnology, College of Physics and Technology, Xinjiang University, Urumqi 830008, China College of Resource and Environment Science, Xinjiang University, Urumqi 830046, China College of Resource and Environment Science, Xinjiang University, Urumqi 830046, China College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China Glycyrrhizic acid (GA) in Glycyrrhiza uralensis (G. uralensis) is physiologically active. In this study, the total DNA of wild G. uralensis was randomly transformed into Hansenula anomala by implantation of low-energy Ar + and N + , to produce five recombinant yeast strains relating to biological synthesis of the GA or Glycyrrhetinic acid (GAs). After culturing in liquid medium for 96 h, the resultant GA, 18 α -GAs and 18 β -Gas were determined by reversed-phase high performance liquid chromatography (RP-HPLC), and the corresponding concentrations were 114.49, 0.56, and 0.81 mg∙L –1 . After one hundred primers were analyzed with random amplified polymorphic DNA (RAPD), the seven different DNA fragments were produced by the N7059 strain of recombined yeasts, and, the polymerase chain reaction (PCR) verified that one of them came from the genome of G. uralensis, indicating a successful transfer of genetic information by ion implantation. Ion implantation total DNA of G. uralensis Transformation Recombinant yeast Glycyrrhizic acid References Wang Y Q , He R X . J Eco Agri , 2004 , 12 : 194 - 195 . (in Chinese) J Eco Agri Liu S J , Jin X , Mao P H . Biotech , 2006 , 16 : 96 - 98 . Biotech Cinatl J , Morgenstern B , Bauer G , et al . Lancet , 2003 , 361 : 2045 - 2046 . Lancet Takei M , Kobayashi M , Li X D , et al . Pathobiolog , 2005 , 72 : 117 - 123 . Pathobiolog Harada S . Biochem J , 2005 , 15 : 191 - 199 . Biochem J Yu Z L . Introduction to ion beam biotechnology . New York : Springer Publishing House , 2006 , 211 - 226 . Introduction to ion beam biotechnology Song D J , Chen R L , Jun R C , et al . Prog Nat Sci , 2001 , 11 : 557 - 560 . Prog Nat Sci Mao P H , Ma X D , Jin X , et al . Acta Microbiologic Sinic , 2007 , 47 : 905 - 909 . Acta Microbiologic Sinic Lü J , Jin X , Mao P H , et al . Appl Biochem Biotechnol , 2009 , 158 : 571 - 581 . Appl Biochem Biotechnol Huang G W , Mao P H , Jin X , et al . Genomics & Appl Biolog , 2009 , 28 : 815 - 820 . Genomics & Appl Biolog Burke D , Dawson D , Stearns T . Methods in yeast genetics . New York : Cold Spring Harbor Lab Press . 2000 , 171 - 172 . Methods in yeast genetics Shao P Z , Cao H . Shanghai : Fudan University Press . 2004 , 49 - 71 . Wu J F . Natural pharmaceutical chemistry , Beijing : Peoples Medical Publishing House . 2003 , 21-36, 125-127, 288-291 . Natural pharmaceutical chemistry Feng H Y , Yu Z L , Chu P K . Mat Sci Eng R , 2006 , 54 : 49 - 120 . Mat Sci Eng R Ro D K , Paradise E M , Ouellet M , et al . Nature , 2006 , 440 : 940 - 943 . Nature 10.13538/j.1001-8042/nst.21.347-351 Supported by National Natural Science Foundation of China (Grant No. 30560182, 30960006) 2010-05-10 2010-12-20 2021-01-19 2010 Nuclear Science and Techniques 06 21