Feasibility of ^99mTc-TRODAT-1 Micro-SPECT imaging of dopamine transporter in animal retinas

Feasibility of 99m Tc-TRODAT-1 Micro-SPECT imaging of dopamine transporter in animal retinas 1 first-author 2 2 2 3 3 1 corresp Corresponding author. E-mail address:xingdliu@yahoo.com Corresponding author. E-mail address:xingdliu@yahoo.com xingdliu@yahoo.com Department of Nuclear Medicine, Huashan Hospital of Fudan University, Shanghai 200040, China Department of Nuclear Medicine, Huashan Hospital of Fudan University, Shanghai 200040, China Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Eye and ENT Hospital of Fudan University, Shanghai 200031, China Eye and ENT Hospital of Fudan University, Shanghai 200031, China In this paper, 99m Tc-TRODAT-1 Micro-SPECT (single-photon emission computed tomography) was used for imaging dopamine transporter (DAT) in retinas and to investigate the changes of DAT in retinas of guinea pigs with form deprivation myopia. Pigmented guinea pigs aged 3 weeks were devided into form deprivation myopia (FDM) group ( n =6) and normal control group ( n =6). The test group wore translucent goggles randomly for 4 weeks, and both groups underwent biometric measurement (refraction and axial length) before and after the experiment. Micro-SPECT retinas imaging was performed at the 4 th week after injection of 99m Tc-TRODAT-1. The retinas were clearly resolved in the images. The ratio of 99m Tc-TRODAT-1 uptake in the myopic retinas (11.55±2.80) was 3.64±1.40 lower than that in the control eye (15.20±1.98), and 2.35±1.05 lower than that in the fellow eyes (13.90±2.04). The results showed that 99m Tc-TRODAT-1 Micro-SPECT eye imaging can be used to trace the distribution and changes of DAT in retina, and DAT in the myopic retinas were lower than that in the normal control eyes and fellow eyes. Micro-SPECT may provide a new approach for further studies on the role of dopamine system in the experimental myopia. Micro-SPECT 99m Tc-TRODAT-1 Dopamine transporter Form deprivation myopia Guinea pig References Howlett M H , McFadden S A . Vision Res , 2006 , 46 : 267 - 283 . Vision Res McFadden S A , Howlett M H , Mertz J R . Vision Res , 2004 , 44 : 643 - 653 . Vision Res Qiao G Y , Hung L F , Kee C S , et al . Invest Ophthalmol Vis Sci , 2004 , 45 : 3361 - 3372 . Invest Ophthalmol Vis Sci Siegwart J T , Norton T T . Invest Ophthalmol Vis Sci , 2002 , 43 : 2067 - 2075 . Invest Ophthalmol Vis Sci Regan A . Exp Eye Res , 2007 , 85 : 15 - 22 . Exp Eye Res Schwarz J , Linke R , Kemer M , et al . Arch Neurol , 2000 , 57 : 205 - 208 . Arch Neurol Liu X D , Guo L P , Jiang C C , et al . Shanghai Med J (in Chinese) , 2002 , 25 : 607 - 609 . Shanghai Med J (in Chinese) Goertzen A L , Jones D W , Seidel J , et al . IEEE Trans Med Imaging , 2005 , 24 : 863 - 867 . IEEE Trans Med Imaging Acton P D , Choi S R , Plössl K , et al . Eur J Nucl Med , 2002 , 29 : 691 - 698 . Eur J Nucl Med Acton P D , Hou C , Kung M P , et al . Eur J Nucl Med , 2002 , 29 : 1508 - 1515 . Eur J Nucl Med Herschman H R . Curr Opin Immunol , 2003 , 15 : 378 - 384 . Curr Opin Immunol Liu C J , Liu X D , Zhang G M , et al . Nucl Sci and Tech 2007 , 18 : 232 - 236 . Nucl Sci and Tech Huang W S , Lin S Z , Lin J C , et al . J Nucl Med , 2001 , 42 : 1303 - 1308 . J Nucl Med Xi X Q , Chu R Y , Zhou X T , et al . Chin Med J , 2002 , 115 : 1027 - 1030 . Chin Med J Wang W F , Kiichi I , Motohiro K , et al . Ophthalmac Res 2004 , 36 : 255 - 263 . Ophthalmac Res 10.1016/S1001-8042(08)60032-9 R817.4 2007-12-02 2008-04-20 2021-01-19 2008 Nuclear Science and Techniques 02 19