Study of calf thymus DNA irradiated in vitro with MeV fluorine ions

Study of calf thymus DNA irradiated in vitro with MeV fluorine ions 1 2 corresp first-author E-mail: miaoqimiaoqi@126.com E-mail: miaoqimiaoqi@126.com miaoqimiaoqi@126.com 2 2 2 1 1 2 Henan Provincial Key Lab of Ionbeam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; Henan Provincial Key Lab of Ionbeam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; Key Laboratory of Heavy Ion Physics, Peking University, MOE, Beijing 100871, China Key Laboratory of Heavy Ion Physics, Peking University, MOE, Beijing 100871, China A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects (induced by heavy ions). In this experiment, the products of calf thymus DNA (CT DNA) irradiated with MeV fluorine ions were analyzed using agarose gel electrophoresis, modified time-of-flight mass spectrometer (MALDI-TOF), and high-performance liquid chromatography (HPLC). The results showed that the molecular mass of the fragments were concentrated around 831 bp with agarose gel electrophoresis, there was no observable product in the range of 1,000-30,000 ( m / q ) using MALDI-TOF, and small biomolecules were separated from the products. The results of this study indicated that the strand breaks of calf thymus DNA induced by MeV fluorine ions were nonrandom. MeV ion Irradiation DNA Damage References Zhou Guangming , Wei Zengquan , Li Wenjian , et al . Acta Biophysica Sinica , 1998 , 14 ( 1 ): 145 - 149 (in Chinese) Acta Biophysica Sinica Zhou Guangming , Li Wenjian , Wang Jufang , et al . Acta Biophysica Sinica , 2000 , 16 ( 1 ): 56 - 61 (in Chinese) Acta Biophysica Sinica Du Yanhua , Huang Shenghai , Tan Zheng , et al . Chinese Science Bulletin , 1999 , 44 ( 1 ): 56 - 61 (in Chinese) Chinese Science Bulletin Du Yanhua , Qiu Guanying , Li Guohong , et al . Acta Biophysica Sinica , 1997 , 13 ( 2 ): 267 - 272 (in Chinese) Acta Biophysica Sinica Wang Xiangqin , Han Jianwei , Yu Zengliang , Acta Biophysica Sinica , 1998 , 14 ( 2 ): 337 - 340 (in Chinese) Acta Biophysica Sinica Li Lingyun , WANG Yugang , XUE Jianming , et al , Nuclear Science and Techniques , 1999 , 10 ( 4 ): 221 - 224 Nuclear Science and Techniques Huang Weidong , Yu Zengliang , Zhang Yuheng . Nuclear Instruments and Methods in Physics Research B , 1998 , 134 : 202 - 208 Nuclear Instruments and Methods in Physics Research B Chatterjee A , Holley W. R ., Int J Quantum Chem , 1991 , 39 ( 5 ): 709 - 727 Int J Quantum Chem Milligan J. R ., Ng J. Y-Y. , Wu C. C.L. , et al . Radiatat Res , 1995 , 143 ( 3 ): 273 - 280 Radiatat Res Denifl S ., Ptasinska S ., Cingel M. , et al . Chem Phys Lett , 2003 , 377 : 74 - 80 Chem Phys Lett Fuciarelli A.F ., Sisk E.C. , Miller J.H. , et al . Int J Radiat Biol , 1994 , 66 ( 5 ): 505 - 509 Int J Radiat Biol 10.1016/S1001-8042(06)60050-X O571.32 + 4,Q691.5,Q523 2005-12-17 2006-10-20 2021-01-19 2006 Nuclear Science and Techniques 05 17