Apoptosis and necrosis of HeLa cells in response to low-energy ion radiation

Apoptosis and necrosis of HeLa cells in response to low-energy ion radiation 1 corresp first-author E-mail: zhangfengqiu@zzu.edu.cn E-mail: zhangfengqiu@zzu.edu.cn zhangfengqiu@zzu.edu.cn 1 1 1 1 1 1 Henan Key Lab of Ionbeam Bioengineering, Zhengzhou University, Zhengzhou 450052, China Henan Key Lab of Ionbeam Bioengineering, Zhengzhou University, Zhengzhou 450052, China The aim of this study was to investigate the damage of low-energy ions to HeLa cells and to particularly examine the relationship between apoptotic and necrotic effects and the low-energy ion radiation. In this study, HeLa cells were irradiated by low-energy ions (30keV N + ) at different doses. The level of apoptosis and necrosis was evaluated using flow cytometry. Since vacuum is required for experimental low-energy ion generation and irradiation, the cells must be placed in vacuum. Mineral oil was used to prevent dehydration of cells. The results show that the apoptotic rate reached 7.09% when the ion implantation dose was 1×10 15 ions/cm 2 ; and when the cells were exposed to and implanted at 2×10 15 ions/cm 2 dose, the apoptotic rate was higher than that at 1×10 15 ions/cm 2 , and the necrotic rate was 15.63%. In addition, the survival fraction gradually decreased with the increase in implantation dose. Some relationships have been found between the radiation-induced apoptosis and the incubated time after irradiation. Low-energy ion Apoptosis Necrosis Flow cytometry References FENG Hui-Yun , YU Li-Xiang , LIU Xing-Hai , et al . Proceedings of the Third National Conference of Ion Beam Bioengineering and the First International Symposium on Ion Beams , Urumqi, China , 2002 : 17 - 21 Yu L D , Viaithong T , Phanchaisri B , Nuclear Instrument and Methods in Physics Research , 2003 , B206 : 586 - 590 Nuclear Instrument and Methods in Physics Research Yu Z , Shao C , Yang J . J. Anhui Agric.Univ (in Chinese) , 1994 , 21 ( 3 ): 260 J. Anhui Agric.Univ (in Chinese) Yu L D , Phanchaisri B , Apavatjrut P , et al . Surface and Coatings Technology , 2002 , 158-159 : 146 - 150 Surface and Coatings Technology Vermes I , Haanen C , Steffens-Nakken H , et al . Journal of Immunological Methods , 1995 , 184 : 39 - 51 Journal of Immunological Methods Sheridan M T , West C M L . Int. J. Radiation Oncology Biol. Phys , 2001 , 50 : 503 - 509 Int. J. Radiation Oncology Biol. Phys Proskuryakov Y S , Konoplyannikov A G , Gabai V L . Experimental Cell Research , 2003 , 283 : 1 - 16 Experimental Cell Research Dewey W C , Ling C C , Meyn R E . Int. J. Radiation Oncology Biol. Phys , 1995 , 33 : 781 - 796 Int. J. Radiation Oncology Biol. Phys Feng Huiyun , Wu Lijun , Xu An , et al . Cryobiology , 2004 , 49 : 241 - 249 Cryobiology Meyn RE , Stephens LC , Voehringer DW , et al . Radiat. Res , 1993 , 136 : 327 - 334 Radiat. Res Zheng Yixin , Oncology (in Chinese) , Beijing : People’s Medical Publishing House (PMPH) , 1999 : 368 Oncology (in Chinese) 10.1016/S1001-8042(06)60041-9 Q345 + .2,Q274 Supported by the National Science and Technique Foundation during the Tenth Five-Year Plan Period of China (2001BA302B-03) . 2005-11-23 2006-08-20 2021-01-19 2006 Nuclear Science and Techniques 04 17