Acute exposure to high-peak-power pulsed microwaves affecting the histamine H₃ receptor expression in rat hippocampus

Acute exposure to high-peak-power pulsed microwaves affecting the histamine H 3 receptor expression in rat hippocampus 1 2 first-author 1 2 2 2 1 corresp Corresponding author. E-mail: yxd@cqnu.edu.cn Corresponding author. E-mail: yxd@cqnu.edu.cn yxd@cqnu.edu.cn Department of Occupational Health, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China; Department of Occupational Health, School of Preventive Medicine, Third Military Medical University, Chongqing 400038, China; Chongqing Key Laboratory of Animal Sciences, School of Biological Science, Chongqing Normal University, Chongqing 400037, China Chongqing Key Laboratory of Animal Sciences, School of Biological Science, Chongqing Normal University, Chongqing 400037, China In the Morris Water Maze test, high-peak-power pulsed microwave (MW)-exposed rats displayed some learning and memory behavior dysfunctions, and their escape time and swimming distance to the submerged platform were longer than those of the sham-exposed rats. To understand the molecular mechanism involved, the reverse transcription-polymerase chain reaction (RT-PCR) and the Western-blotting technique were used for investigating the mRNA and protein expression patterns of the histamine H 3 receptor (H 3 R) in rat hippocampus. High-peak-power pulsed microwave-exposure did not remarkably lead to the change in expression of H 3 R mRNA in rat hippocampi; however, it promoted the up-regulatory expression of the H 3 R protein, which was possibly triggered through the mitogen-activated protein kinase (MAPK) pathways. Therefore, further investigation of the molecular mechanism of the MW effects on the learning and memory behaviors is required. Histamine H 3 -receptor Rat hippocampus Microwave Expression References D’Andrea J A , Chou C K , Johnston S A , et al . Bioelectromagnetics (supp.) , 2003 , 6 : s107 - s147 . Bioelectromagnetics (supp.) Vander V A , Duhamel F , IEEE Trans Microw Theory Tech , 1996 , 44 : 1898 - 1909 . IEEE Trans Microw Theory Tech Wang B , Lai H . Bioelectromagnetics , 2000 , 21 : 52 - 56 . Bioelectromagnetics Alves-Rodrigues A , Timmerman H , Willems E , et al . Brain Research , 1998 , 788 : 179 - 186 . Brain Research Bekkers J M , Science , 1993 , 261 : 104 - 106 . Science Philippu A , Prast H , Behavioural Brain Research , 2001 , 124 : 151 - 159 . Behavioural Brain Research Orsetti M , Ghi P , Carlo GD . Behavioural Brain Research , 2001 , 124 : 235 - 242 . Behavioural Brain Research Toyota H , Dugovic C , Koehl M , et al . Mol Pharmacol , 2002 , 62 : 389 - 397 . Mol Pharmacol Leurs R , Blandina P , Tedford C , et al . Trends Pharmacol Sci , 1998 , 19 : 177 - 183 . Trends Pharmacol Sci Arrange J M , Garbarg M , Schwartz J C . Nature , 1983 , 302 : 832 - 837 . Nature Shin N , Coates E , Murgolo N J , et al . Mol Pharmacol , 2002 , 62 : 38 - 47 . Mol Pharmacol Hill S J , Ganellin C R , Timmerman H , et al . Pharmacol Rev , 1997 , 49 : 253 - 278 . Pharmacol Rev Lovenberg T W , Pyati J , Chang H , et al . J Pharmacol Exp Thr , 2000 , 293 : 771 - 778 . J Pharmacol Exp Thr Drutel G , Peitsaro N , Karlstedt K , et al . Mol Pharmacol , 2001 , 59 : 1 - 8 . Mol Pharmacol Jauchem J R , Frei M R . Aviation, Space and Environmental Medicine , 1995 , 66 : 992 - 997 . Aviation, Space and Environmental Medicine Cobb B L , Jauchem J R , Adair E R . Bioelectromagnetics , 2004 , 25 : 49 - 57 . Bioelectromagnetics Blandina P , Giorgetti M , Bartolini L , et al . Br J Pharmacol , 1996 , 119 : 1656 - 1664 . Br J Pharmacol Fox G B , Pan J B , Radek R J , et al . JPET , 2003 , 305 : 897 - 908 . JPET 10.1016/S1001-8042(06)60042-0 R122,R122.4 2006-03-21 2006-08-20 2021-01-19 2006 Nuclear Science and Techniques 04 17