Effect of N⁺ beam exposure on the activities of Mn-SOD and catalase in Deinococcus radiodurans

Effect of N + beam exposure on the activities of Mn-SOD and catalase in Deinococcus radiodurans 1 corresp first-author E-mail: zlyu@mail.ipp.ac.cn or cdhu@mail.ipp.ac.cn E-mail: zlyu@mail.ipp.ac.cn or cdhu@mail.ipp.ac.cn zlyu@mail.ipp.ac.cn 1 1 1 1 1 1 1 Center of Fengle Ion Beam Bioengineering, Institute of Plasma Physics, the Chinese Academy of Sciences, Hefei 230031 Center of Fengle Ion Beam Bioengineering, Institute of Plasma Physics, the Chinese Academy of Sciences, Hefei 230031 Though the radiation-resistant bacteria Deinococcus radiodurans (D. radiodurans) have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to oxidative stress are poorly understood. In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced in bacteria (D.radiodurans AS1.633) and their change caused by 20keV N + beam exposure were examined. Results showed that the activities of the enzymes were increased in the case of N + beam exposure from 8 × 10 14 ions/cm 2 to 6 × 10 15 ions/cm 2 . In addition, the treatment of H 2 O 2 and [CHCl 3 +CH 3 CH 2 OH] and the measurement of absorption spectrum showed that the increase of whole SOD activity resulted from inducible activities of Mn-SOD in (a sub-type) D.radiodurans AS1.633. These results suggested that these bacteria possess inducible defense mechanisms against the deleterious effects of oxidization. N + beam exposure Deinococcus radiodurans AS1.633 Superoxide dismutase Catalase References Brooks B W , Murray R G E . Int J Syst Bacteriol , 1981 31 : 353 ~ 360 Int J Syst Bacteriol Masters C I , Minton K W . Plasmoid , 1992 , 28 : 258 ~ 261 Plasmoid Ghalib H A , Mackay M W et al . Gene , 1985 , 33 : 305 ~ 311 Gene Huang Y , Anderson R . J Bacteriol , 1995 , 177 ( 9 ): 2564 ~ 2571 J Bacteriol Tempest P R and Moseley B E B . Molec Gen Genet , 1980 , 179 : 191 ~ 199 Molec Gen Genet Lennon E , Mintin K W . J Bacteriol . 1990 , 172 ( 6 ): 2955 ~ 2961 J Bacteriol Daly M J , Ouyang L . J Bacteriol , 1994 , 176 ( 24 ): 7506 ~ 7515 J Bacteriol Carroll J D , Daly M J , Minton K W . J Bacteriol , 1996 , 178 ( 1 ): 130 ~ 135 J Bacteriol Daly M J , Ouyang L . J Bacteriol , 1994 , 176 ( 12 ): 35508 ~ 3517 J Bacteriol Fridovich I . Science , 1978 , 201 : 875 ~ 880 Science Repine J E et al . Proc Natl Acad Sci U S A , 1981 , 78 : 1001 ~ 1003 Proc Natl Acad Sci U S A Yu Z L et al . Nucl Instr Meth , 1991 , B59/60 : 705 ~ 708 Nucl Instr Meth Yu Z L et al . Nucl Instr Meth , 1993 , B80/81 : 1328 ~ 1331 Nucl Instr Meth Yu Z L . Radiat Phys Chem , 1994 , 43 ( 4 ): 349 ~ 351 Radiat Phys Chem Wang P . Can J Bacteriol , 1994 , 41 : 170 ~ 176 Can J Bacteriol Chou F I , Tan S T . J Bacteriol , 1990 , 172 ( 4 ): 2029 ~ 2035 J Bacteriol Yu Z L. Deng J G et al . Nucl Instr Meth , 1991 , 59/60 : 705 ~ 708 Nucl Instr Meth Yu Z L , Yang J B et al . Studies of ion implantation effect on biology in China, China Nuclear Science and Technology Report (in Chinese), 1993 CNIC00746, ASIPP0036 Studies of ion implantation effect on biology in China, China Nuclear Science and Technology Report (in Chinese), 1993 CNIC00746, ASIPP0036 Yu Z L , Yang J B et al . Nucl Instr Meth , 1993 , B80/81 : 1328 ~ 1331 Nucl Instr Meth Wang X Q , Han J W et al . Viva Origino , 1990 , 26 ( 2 ): 109 ~ 116 Viva Origino Beauchamp C , Fridiovich I , Anal Biochern , 1971 , 44 : 276 ~ 287 Anal Biochern Beers R F , Sizer L W . J Biol Chem , 1952 , 196 : 133 ~ 140 J Biol Chem Li P F , Fang Y Z . J Biochem Sinica , 1993 , 417 ~ 423 J Biochem Sinica Fang Y Z . Free radicals and enzyme and their application in biology and medicine . Beijing : Science and Technology Press , 1994 , 71 ~ 109 Free radicals and enzyme and their application in biology and medicine Luo G H . Prog Biochem Biophys , 1996 , 23 ( 4 ): 356 ~ 359 Prog Biochem Biophys Luo G H . Acta Phytophysiol Sinica , 1994 , 10 ( 2 ): 175 ~ 180 Acta Phytophysiol Sinica Q813.5, Q814, Q691.5 Supported by the National Natural Science Foundation of China (No. 19605005) 2000-05-07 2000-11-01 2021-02-05 2000 Nuclear Science and Techniques 4 11