Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody

Zhaoxiang GU; Xiangtong LIN; Fangping XUE; Jinming ZHANG

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Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody

Updated：2021-02-05

- Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody
- Nuclear Science and Techniques Vol. 10, Issue 4, (1999)
- Affiliations：
  
  1.Huashan Hospital, Shanghai Medical University, Shanghai 200040
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Zhaoxiang GU, Xiangtong LIN, Fangping XUE, et al. Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody. [J]. Nuclear Science and Techniques 10(4):193-196(1999)
DOI：

Zhaoxiang GU, Xiangtong LIN, Fangping XUE, et al. Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody. [J]. Nuclear Science and Techniques 10(4):193-196(1999) DOI：

摘要

Abstract

The human granulocytes were isolated. Using hybridoma techniques, a hybridoma cell line (HSN) producing monoclonal antibody (McAb) against human granulocyte was obtained. The antibody belonged to IgG1 subclass. It was confirmed by immunohistochemical tests that HSN reacted selectively not only with human granulocytes, but also with their bone marrow precursors. Whereas human lymphocytes and red blood cells retained negative in the tests. No cross-reaction was observed with the peripheral blood cells in other animals. Its affinity constant was 5.7×10,8,L/mol, and the number of epitopes per granulocyte was 4.7×10,5,. Monoclonal antibody displayed no loss of immunoreactivity after labelled with ,99m,Tc.

关键词

Keywords

Monoclonal antibodyHuman granulocytesTechnetium-99m labellingHybridoma

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Related Institution

Medical Isotopes Research Center and Department of Radiation Medicine, School of Basic Medical Sciences, Peking University

Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences

Shanghai No.6 People's Hospital

Institute of Nuclear Science and Technology, Sichuan Union University

Institute of Cancer Research, West China University of Medical Sciences

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