Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of <italic style="font-style: italic">Bacillus subtilis</italic> Bac01

Jie LÜ; Peihong MAO; Xiang JIN; Long YU; Hanjie YING

doi:10.13538/j.1001-8042/nst.20.271-276

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Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

LOW ENERGY ACCELERATOR AND RADIATION APPLICATIONS | Updated：2021-01-29

- Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01
- Nuclear Science and Techniques Vol. 20, Issue 5, (2009)
- Affiliations：
  
  1.College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 210009 Nanjing, China
  2.Center of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, 830008 Urumqi, China
- Author bio：
  
  Corresponding author. E-mail address: yinghanjie@njut.edu.cn
- Funds：
- DOI：10.13538/j.1001-8042/nst.20.271-276
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Jie LÜ, Peihong MAO, Xiang JIN, et al. Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01. [J]. Nuclear Science and Techniques 20(5):271-276(2009)
DOI：

Jie LÜ, Peihong MAO, Xiang JIN, et al. Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01. [J]. Nuclear Science and Techniques 20(5):271-276(2009) DOI： 10.13538/j.1001-8042/nst.20.271-276.

摘要

Abstract

Bacillus subtilis, Bac01 was mutated by 15 keV N,+, ions of 1.5×10,16, cm,-2,. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from,B. subtilis, Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.

关键词

Keywords

Ion irradiationBacillus subtilisEndo-1;4-β-D-glucanaseActivityGene mutation

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Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

DOI：10.13538/j.1001-8042/nst.20.271-276

摘要

Abstract

关键词

Keywords

references