Fluorimetric study on the interaction between fluoresceinamine and bovine serum albumin

Yu-Shuang LIU; Ping ZHANG; Rui-Bo ZHONG; Zhi-Jun BAI; Jun GUO; Guo-Fen ZHAO; Feng ZHANG

doi:10.13538/j.1001-8042/nst.26.030505

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Fluorimetric study on the interaction between fluoresceinamine and bovine serum albumin

NUCLEAR PHYSICS AND INTERDISCIPLINARY RESEARCH | Updated：2021-01-20

- Fluorimetric study on the interaction between fluoresceinamine and bovine serum albumin
- Nuclear Science and Techniques Vol. 26, Issue 3, Article number: 030505(2015)
- Affiliations：
  
  1.School of Life Science, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot 010018, China.
- Author bio：
  
  Corresponding author, fengzhang1978@hotmail.com
- Funds：
- DOI：10.13538/j.1001-8042/nst.26.030505
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Yu-Shuang LIU, Ping ZHANG, Rui-Bo ZHONG, et al. Fluorimetric study on the interaction between fluoresceinamine and bovine serum albumin. [J]. Nuclear Science and Techniques 26(3):030505(2015)
DOI：

Yu-Shuang LIU, Ping ZHANG, Rui-Bo ZHONG, et al. Fluorimetric study on the interaction between fluoresceinamine and bovine serum albumin. [J]. Nuclear Science and Techniques 26(3):030505(2015) DOI： 10.13538/j.1001-8042/nst.26.030505.

摘要

Abstract

Fluorescence spectroscopy was employed to investigate the interaction between fluorophore fluoresceinamine (FA) and bovine serum albumin (BSA) under physiological conditions. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by FA is a result of the formation of a BSA-FA complex. Fluorescence quenching constants were determined using the modified Stern-Volmer equation to provide a measure of the binding affinity between FA and BSA. The results of the thermodynamic parameters Δ ,G, Δ ,H, and Δ ,S, at different temperatures indicated that several kinds of interactions, except for the electrostatic interactions play cooperative roles in BSA-FA association. Furthermore, the conformation of BSA upon interaction with FA was also studied by synchrotron fluorescence spectroscopy.

关键词

Keywords

Bovine serum albuminFluoresceinamineFluorescence quenchingBinding constantProtein conformation

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