Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–<sup>99m</sup>Tc labeling of graphene oxide nanosheets

Da-wei JIANG; Cheng PENG; Yan-Hong SUN; Li-Na JIA; Jian-Bo LI; Lan ZHANG

doi:10.13538/j.1001-8042/nst.26.040301

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Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–^99mTc labeling of graphene oxide nanosheets

NUCLEAR CHEMISTRY, RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE | Updated：2021-01-20

- Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–^99mTc labeling of graphene oxide nanosheets
- Nuclear Science and Techniques Vol. 26, Issue 4, Article number: 040301(2015)
- Affiliations：
  
  1.Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  2.Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, China
- Author bio：
  
  Corresponding author, zhanglan@sinap.ac.cn
- Funds：
- DOI：10.13538/j.1001-8042/nst.26.040301
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Da-wei JIANG, Cheng PENG, Yan-Hong SUN, et al. Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–^99mTc labeling of graphene oxide nanosheets. [J]. Nuclear Science and Techniques 26(4):040301(2015)
DOI：

Da-wei JIANG, Cheng PENG, Yan-Hong SUN, et al. Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–^99mTc labeling of graphene oxide nanosheets. [J]. Nuclear Science and Techniques 26(4):040301(2015) DOI： 10.13538/j.1001-8042/nst.26.040301.

摘要

Abstract

Graphene oxide (GO) nanosheets possess several advantages, such as a large surface, outstanding bio-compatibility, and straightforward chemical modification capability. They also have great potential as a drug-carrier. In this article, we radiolabeled GO nanosheets with ,99m,Tc, which satisfies the potential needs of micro-SPECT imaging probes in pre-clinical and clinical research. GO nanosheets were synthesized through the modified Hummers’ method, then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA (1,4,7,10-tetraazacyclododecane-,N,N’,N",N" ’,-tetraacetic acid) and functionalized with an alkynyl group by means of click chemistry. Then through the addition and reduction of technetium-99m, the ,99m,Tc-DOTA-GO were attained. DOTA-conjugated GOs with lateral dimensions of 500–600 nm were synthesized. Both atomic force microscopy (AFM) and FT-IR were performed to characterize the GO-DOTA. Labeling efficiency of GO-DOTA with ,99m,Tc was ,>,90% and radiochemical purities were ,>,96% with purification. We successfully synthesized graphene oxide derivatives, DOTA-conjugated GOs, via Click Chemistry, and it was labeled with ,99m,Tc for SPECT imaging. High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.

关键词

Keywords

Graphene oxide nanosheets99mTc labelingClick chemistry

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Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–99mTc labeling of graphene oxide nanosheets

DOI：10.13538/j.1001-8042/nst.26.040301

摘要

Abstract

关键词

Keywords

references

Study on technetium-99m labeling of graphene oxide nanosheets through click chemistry–^99mTc labeling of graphene oxide nanosheets