Structural biology investigation of EMF effect on insulin in solution

Yahui LIU; Yongbin CHEN; Chunyan XU; Shenglong XU; Bo SUN; Huan ZHOU; Lihua SUN; Feng YU; Lin TANG; Guozhen GUO; Jianhua HE

doi:10.13538/j.1001-8042/nst.22.134-138

Your Location：

Home >

Browse articles >

Structural biology investigation of EMF effect on insulin in solution

SYNCHROTRON TECHNOLOGY AND APPLICATIONS | Updated：2021-01-29

- Structural biology investigation of EMF effect on insulin in solution
- Nuclear Science and Techniques Vol. 22, Issue 3, (2011)
- Affiliations：
  
  1.Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  3.Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China
- Author bio：
  
  Corresponding author. E-mail address: kangchu@fmmu.edu.cn (GUO Guozhen), hejianhua@sinap.ac.cn (HE Jianhua)
  
  C
  o
  r
  r
  e
  s
  p
  o
  n
  d
  i
  n
  g
  
  a
  u
  t
  h
  o
  r
  .
  
  E
  -
  m
  a
  i
  l
  
  a
  d
  d
  r
  e
  s
  s
  :
  
  k
  a
  n
  g
  c
  h
  u
  @
  f
  m
  m
  u
  .
  e
  d
  u
  .
  c
  n
  
  (
  G
  U
  O
  
  G
  u
  o
  z
  h
  e
  n
  )
  ,
  
  h
  e
  j
  i
  a
  n
  h
  u
  a
  @
  s
  i
  n
  a
  p
  .
  a
  c
  .
  c
  n
  
  (
  H
  E
  
  J
  i
  a
  n
  h
  u
  a
  )
  Corresponding author. E-mail address: kangchu@fmmu.edu.cn (GUO Guozhen), hejianhua@sinap.ac.cn (HE Jianhua)
  
  C
  o
  r
  r
  e
  s
  p
  o
  n
  d
  i
  n
  g
  
  a
  u
  t
  h
  o
  r
  .
  
  E
  -
  m
  a
  i
  l
  
  a
  d
  d
  r
  e
  s
  s
  :
  
  k
  a
  n
  g
  c
  h
  u
  @
  f
  m
  m
  u
  .
  e
  d
  u
  .
  c
  n
  
  (
  G
  U
  O
  
  G
  u
  o
  z
  h
  e
  n
  )
  ,
  
  h
  e
  j
  i
  a
  n
  h
  u
  a
  @
  s
  i
  n
  a
  p
  .
  a
  c
  .
  c
  n
  
  (
  H
  E
  
  J
  i
  a
  n
  h
  u
  a
  )
- Funds：
- DOI：10.13538/j.1001-8042/nst.22.134-138 CLC：
- Published：20 June 2011，
  
  Received：23 February 2011，
Scan for full text
Cite this article
Yahui LIU, Yongbin CHEN, Chunyan XU, et al. Structural biology investigation of EMF effect on insulin in solution. [J]. Nuclear Science and Techniques 22(3):134-138(2011)
DOI：

Yahui LIU, Yongbin CHEN, Chunyan XU, et al. Structural biology investigation of EMF effect on insulin in solution. [J]. Nuclear Science and Techniques 22(3):134-138(2011) DOI： 10.13538/j.1001-8042/nst.22.134-138.

Structural biology investigation of EMF effect on insulin in solution 1 2 equal first-author 3 equal 1 3 1 1 1 1 1 3 corresp Corresponding author. E-mail address: kangchu@fmmu.edu.cn (GUO Guozhen), hejianhua@sinap.ac.cn (HE Jianhua) Corresponding author. E-mail address: kangchu@fmmu.edu.cn (GUO Guozhen), hejianhua@sinap.ac.cn (HE Jianhua) kangchu@fmmu.edu.cn 1 corresp Corresponding author. E-mail address: kangchu@fmmu.edu.cn (GUO Guozhen), hejianhua@sinap.ac.cn (HE Jianhua) Corresponding author. E-mail address: kangchu@fmmu.edu.cn (GUO Guozhen), hejianhua@sinap.ac.cn (HE Jianhua) kangchu@fmmu.edu.cn Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Graduate University of Chinese Academy of Sciences, Beijing 100049, China Graduate University of Chinese Academy of Sciences, Beijing 100049, China Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China It was reported that the α-helix of protein molecules could be destroyed when they were exposed to the electromagnetic field (EMF) and finally the signal transduction could be affected. To study this effect, one signal molecule, insulin, was exposed to electromagnetic fields at different combinations of the field strength, repetition rate and exposure time. For the first time, structural biology approach was used to detect the EMF effect. The results of a series of measurements on the interaction of electromagnetic fields with insulin in vitro are described. Under our experimental conditions, no effects of electromagnetic field exposure were observed on the molecular conformation. Electromagnetic field Insulin Macromolecular crystallography Signal transduction References Haberdit W . Nature , 1967 , 213 : 72 - 73 . Nature Goodman R , Bassett C , Henderson A . Science , 1983 , 220 : 1283 - 1285 . Science Goodman E M , Greenebaum B , Marron M T . Int Rev Cytol , 1995 , 158 : 279 - 338 . Int Rev Cytol Levin M , Ernst S . Bioelectromagnetics , 1995 , 16 : 231 - 240 . Bioelectromagnetics George I , Geddis M S , Goodman R . J Cell Physiol , 2008 , 216 : 816 - 823 . J Cell Physiol Reinbold K A , Pollack S R . Bioelectromagnetics , 1997 , 18 : 203 - 214 . Bioelectromagnetics Merla C , Liberti M , Apollonio F . Mircrowace Theory Tech , 2010 , 3 : 691 - 698 . Mircrowace Theory Tech Malka N. Halgamuge , Bertil R R . Environ Eng Sci , 2009 , 26 : 1473 - 1480 . Environ Eng Sci Karabakhtsian R , Broude N , Shalts N . FEBS Lett , 1994 , 349 : 1 - 6 . FEBS Lett Lindstrom E , Berglund A , Mild K H . FEBS Lett , 1995 , 370 : 118 - 122 . FEBS Lett Luciana D , Luigi A . Miron , 2005 , 36 : 195 - 217 . Miron Simko M , Mattsson M O . J cell biochem , 2004 , 93 : 83 - 92 . J cell biochem Massot O , Grimaldi B , Bailly J M . Brain Res , 2000 , 858 : 143 - 150 Brain Res Zhou J L , Li C L , Yao G D . Bioelectromagnetics , 2002 , 23 : 339 - 346 . Bioelectromagnetics Verma S P , Goldner R B . Bioelectromagnetics , 1996 , 17 : 33 - 36 . Bioelectromagnetics Li X , Chen S D , Wang L Y . Spectrosc Spect Anal , 2001 , 21 : 406 - 408 . Spectrosc Spect Anal Yan Z , Chen S D , Qiao D J . Spectrosc Spect Anal , 2008 , 28 : 1343 - 1347 . Spectrosc Spect Anal Mannerling A C , Simko M , Mild K H . Radiat Environ Biophy , 2010 , 49 : 731 - 741 . Radiat Environ Biophy Otwinowski Z , Minor W . Method Enzymol , 1997 , 276 : 307 - 326 . Method Enzymol Bailey S . Acta Crystallogr D , 1994 , 50 : 760 - 763 . Acta Crystallogr D Mayer J P , Zhang F , DiMarchi RD . Biopolymers , 2007 , 88 : 687 - 713 . Biopolymers Murrayrust J , Mcleod A N , Wood S P . Bioessays , 1992 , 14 : 325 - 331 . Bioessays Shi C , Chang W H , Chu P . J Bone Miner Res , 2002 , 17 : M238 . J Bone Miner Res Chen G , Upham B L , Sun W . Environ Health Perspect , 2000 , 108 : 967 - 972 . Environ Health Perspect Harris P , Lamb J , Heaton B . Electromagn fields , 2002 , 2 : 3 - 3 . Electromagn fields Korzhsleptsova I L , Lindstrom E , Mild K H . FEBS Lett , 1995 , 359 : 151 - 154 . FEBS Lett Lange S , Richard D , Viergutz T . Radiat Environ Biophys , 2002 , 41 : 131 - 137 . Radiat Environ Biophys Verheyen G R , Pauwels G , Verschaeve L . Bioelectromagnetics , 2003 , 24 : 160 - 164 . Bioelectromagnetics 10.13538/j.1001-8042/nst.22.134-138 Supported by National Natural Science Foundation of China (No.10774155, No.30901215, No.30972430, No.30870576, No.60971055, No. 31000389) and Grant from Shanghai Institute of Applied Physics, Chinese Academy of Sciences (No. O95501C061) . 2011-02-23 2011-06-20 2021-01-19 2011 Nuclear Science and Techniques 03 22

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

The protein complex crystallography beamline (BL19U1) at the Shanghai Synchrotron Radiation Facility

Upgrade of macromolecular crystallography beamline BL17U1 at SSRF

The macromolecular crystallography beamline of SSRF

Related Author

Jian-Chao Tang

Wei-Zhe Zhang

Si-Sheng Wang

Zhi-Jun Wang

Wen-Ming Qin

Jian-Hua He

Zhi-Jun Wang

Qi-Sheng Wang

Related Institution

National Facility for Protein Science in Shanghai, Shanghai Advanced Research Institute (Zhangjiang Laboratory), Chinese Academy of Sciences

Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute (Zhangjiang Laboratory), Chinese Academy of Sciences

University of Chinese Academy of Sciences

⁰