Preparation, quality control and biodistribution of [⁶¹Cu]-doxorubicin for PET imaging

Preparation, quality control and biodistribution of [ 61 Cu]-doxorubicin for PET imaging 1 corresp first-author Corresponding author. E-mail address: ajalilian@nrcam.org Corresponding author. E-mail address: ajalilian@nrcam.org ajalilian@nrcam.org 2 2 1 1 Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School, Karaj, Iran Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School, Karaj, Iran Department of Nuclear Engineering, School of Engineering, Shiraz University, Shiraz, Iran Department of Nuclear Engineering, School of Engineering, Shiraz University, Shiraz, Iran This work was conducted for radiolabeling of an anticancer antibiotic, i.e. doxorubicin with 61 Cu for production of possible tracer used in PET oncology. 61 Cu was prepared with natural zinc target and 22 MeV150 µA protons via nat Zn( p , xn ) 61 Cu reaction with a yield of 123.2 MBq·µA –1 ·h –1 . Optimization reactions were performed for pH, temperature and concentration. Biodistribution of the tracer was studied in normal and fibrosarcoma bearing mice. At the optimized conditions, ITLC showed that radiochemical purity was over 97% with a specific activity of 2.22×10 3 MBq ·mmol -1 ·L -1 . This was kept unchanged even with presence of human serum as well as room temperature for 5 h. Biodistribution of the tracer in fibrosarcoma bearing mice demonstrated significant tumor uptake after 2 h. This tracer can be used in the detection of various tumors responding to doxorubicin chemotherapy using PET scan and/or determination of tumor therapy response to doxorubicin chemotherapy. Copper-61 Doxorubicin Radiolabeling Biodistribution References Jalilian A R , Mirsadeghi L , Yari-Kamrani Y , et al . J Radioanal Nucl Chem , 2007 , 274 : 563 - 568 . J Radioanal Nucl Chem Rowshanfarzad P , Jalilian A R , Kyomarsi M , et al . Nukleonika , 2006 , 51 : 209 - 215 . Nukleonika Jager P L , de Korte M A , Lub-de Hooge M N , et al . Cancer Imag , 2005 , 23 : AS27 - 32 . Cancer Imag Mccarthy D W , Bass L A , Cutler P D , et al . Nucl Med Biol , 1999 , 26 : 351 - 358 . Nucl Med Biol Ma D F , Lu T , Overstreet D E , et al . Nucl Med Biol , 2002 , 29 : 91 - 105 . Nucl Med Biol Fukumura T , Okada K , Szelecsényi F , et al . Radiochimica Acta , 2004 , 92 : 209 - 214 . Radiochimica Acta Jalilian A R , Rowshanfarzad P , Sabet M . Appl Radiat Isot , 2006 , 64 : 337 - 341 . Appl Radiat Isot Lewis J S , Sharp T L , Laforest R , et al . J Nucl Med , 2001 , 42 : 655 - 661 . J Nucl Med Pharmacia Company . Mithramycin(doxorubicin), drug information inlet , Millan, Italy , 2007 . Mithramycin(doxorubicin), drug information inlet Fischer J G , Tackett R L , Howerth E W , et al . J Nutr , 1992 , 122 : 2128 - 2137 . J Nutr Monti E , Paracchini L , Piccinini F , et al . 1990 , 25 : 333 - 6 . Nikolis N , Methenitis C , Pneumatikakis G , et al . J Inorg Biochem , 2002 , 89 : 131 - 141 . J Inorg Biochem Mizutani H , Oikawa S , Hiraku Y . Cancer Sci , 2003 , 94 : 686 - 691 . Cancer Sci Feng M , Yang Y . Spectrochim Acta A Mol Biomol Spectrosc , 2000 , 56 : 581 - 587 . Spectrochim Acta A Mol Biomol Spectrosc Tachibana M , Iwaizumi M , Tero-Kubota S . J Inorg Biochem , 1987 , 30 : 133 - 140 . J Inorg Biochem Jalilian A R , Sabet M , Rowshanfarzad P , et al , J Radioanal Nucl Chem , 2006 , 269 : 147 - 154 . J Radioanal Nucl Chem Jalilian A R , Shanesazzadeh S , Rowshanfarzad P , et al . J Nucl Sci Tech , 2008 , 19 ( 3 ): 159 - 164 . J Nucl Sci Tech Jalilian A R , Rostampour N , Rowshanfarzad P , et al . Acta Pharm , 2009 , 59 : 45 - 55 . Acta Pharm Katzung G A . Principles of Pharmacology, Anticancer Antibiotics , 1922 . Principles of Pharmacology, Anticancer Antibiotics Lugtenburg P J , Sonneveld P . Curr Oncol Rep , 2008 , 10 : 412 - 419 . Curr Oncol Rep Rowshanfarzad P , Sabet M , Jalilian A R , et al . Appl Radiat Isot , 2006 , 64 : 1563 - 1573 . Appl Radiat Isot Blann M , Bislinghoff J , Code Alice/Livermore 91, Lawrence Livermore National Laboratory, Internal Report, UCID-19614 , 1991 . Code Alice/Livermore 91, Lawrence Livermore National Laboratory, Internal Report, UCID-19614 Ziegler J F , Biersack J P , Littmark U . The code of SRIM-the stopping and range of ions in matter(a software), Version 2000.XX , 2000 . The code of SRIM-the stopping and range of ions in matter(a software), Version 2000.XX Weisberg A M . Gold Plating ( 9 th ed.), U.S.A : ASM International , 1990 : 247 . Gold Plating Schwarzbach R , Zimmerman K , Bläuenstein P , et al . Appl Radiat Isot , 1995 , 46 : 329 - 336 . Appl Radiat Isot Marczenko Z . Spectrophotometric determination of elements ( 4 th ed.), New York : John Wiley & Sons Inc , 1976 : 601 . Spectrophotometric determination of elements Marczenko Z . Spectrophotometric determination of elements ( 4 th ed.). New York : John Wiley & Sons Inc , 1976 : 281 . Spectrophotometric determination of elements Jaaskela-Saari H A , Kairemo K J , Ramsay H A . Int J Radiat Biol , 1998 , 73 : 565 - 570 . Int J Radiat Biol DiGiovanni J , Rymer J , Slaga T J , et al . Carcinogenesis . 1982 , 3 : 371 - 375 . Carcinogenesis Creasey W A , McIntosh L S , Brescia T , et al . Cancer Res , 1976 , 36 : 216 - 221 . Cancer Res 10.13538/j.1001-8042/nst.20.157-162 2008-12-31 2009-06-20 2021-01-19 2009 Nuclear Science and Techniques 03 20