Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01 1 2 first-author 2 2 1 1 corresp Corresponding author. E-mail address: yinghanjie@njut.edu.cn Corresponding author. E-mail address: yinghanjie@njut.edu.cn yinghanjie@njut.edu.cn College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 210009 Nanjing, China College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 210009 Nanjing, China Center of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, 830008 Urumqi, China Center of Ion Beam Biotechnology, College of Physics Science and Technology, Xinjiang University, 830008 Urumqi, China Bacillus subtilis Bac01 was mutated by 15 keV N + ions of 1.5×10 16 cm -2 . The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase. Ion irradiation Bacillus subtilis Endo-1;4- β -D-glucanase Activity Gene mutation References Gao P J . Prog Natl Sci , 2003 , 13 ( 1 ): 21 - 29 . Prog Natl Sci Sun Y , Jin X , Mao P H . Biotechnology , 2005 , 15 ( 3 ): 87 - 89 . Biotechnology Muller H J . Science , 1927 , 66 : 84 - 87 . Science Gu S B , Yao J M , Yuan Q P , et al . Appl Microbiol Biotechnol , 2006 , 72 : 456 - 461 . Appl Microbiol Biotechnol Yu Z L , He J J , Deng J G , et al . Anhui Agricult Sci , 1989 , 28 : 12 - 16 Anhui Agricult Sci Vilaithonga T , Yu L D , Apavatjrutb P , et al . Phys Chem , 2004 , 71 : 927 - 935 . Phys Chem Yu L , Yu Z L . High Technol Lett , 2002 , 12 : 41 - 46 . High Technol Lett Yuan C L , Yao J M , Wang J , et al . J Radiat Res Process , 2003 , 21 : 237 - 242 . J Radiat Res Process Wu L F , Yu Z L . Radiat Environ Biophys , 2001 , 40 : 53 - 57 . Radiat Environ Biophys Wu Y J , Wang X D , Liu G F , et al . J Nucl Agric Sci , 1990 , 11 : 154 - 156 . J Nucl Agric Sci Feng H Y , Yu Z L , Chu P K . Mat Sci Eng R , 2006 , 54 : 49 - 120 . Mat Sci Eng R Jung H C , Park J H , Park S H , et al . Enzyme Microb Technol , 1998 , 22 ( 3 ): 48 - 54 . Enzyme Microb Technol Liu M , Wang J , Liu J , Yao , et al . Ann. Microbiol , 2006 , 56 : 41 - 45 . Ann. Microbiol Lü J , Jin X , Mao P H , et al . Appl Biochem Biotechnol , DOI 10.1007/s12010-009-8597-9 http://doi.org/10.1007/s12010-009-8597-9 . Appl Biochem Biotechnol Wang Y J , Yuan H , Wang J , et al . Bioresour Technol , 2009 , 100 : 345 - 349 . Bioresour Technol Shao C L , Yu Z L . Radiat Phys Chem , 1994 , 44 ( 6 ): 652 - 654 . Radiat Phys Chem Huang W D , Yu Z L , Jiang X Y . Radiat Phys Chem , 1996 , 48 ( 3 ): 319 - 323 . Radiat Phys Chem Shao C L , Yu Z L . Radiat Phys Chem , 1997b , 50 ( 6 ): 595 - 599 . Radiat Phys Chem Hilden L , Johansson G , Biotechnol Lett , 2004 , 26 : 1683 - 1693 . Biotechnol Lett Takashima S , Ohno M , Hidaka M , et al . FEBS Lett , 2007 , 581 : 5891 - 5896 . FEBS Lett 10.13538/j.1001-8042/nst.20.271-276 Supported by the Natural Science Foundations of Xinjiang (No. 2009211B06) and Xinjiang University (No. QN070109) 2009-06-24 2009-10-20 2021-01-19 2009 Nuclear Science and Techniques 05 20