Assessment of biological changes in wheat seedlings induced by ¹²C⁶⁺-ion irradiation

Assessment of biological changes in wheat seedlings induced by 12 C 6+ -ion irradiation 1 2 3 first-author 2 1 1 4 corresp Corresponding author, E-mail: zhujl@gsau.edu.cn Corresponding author, E-mail: zhujl@gsau.edu.cn zhujl@gsau.edu.cn College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China Key Laboratory of Grassland-Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China Key Laboratory of Grassland-Ecosystem, Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou 730070, China The present study was designed to evaluate the effect of 12 C 6+ ion beam (10~80 Gy) on biological changes of wheat seedlings. Reactive oxygen species (ROS)-related biomarkers and the quantification of plant survival and growth were examined at 10 day after carbon ions irradiation (LET: 30.8 keV/μm). The results showed that heavy ions obviously enhanced ROSs reflected by the production of O 2 - and H 2 O 2 as well as TBARS, and treatment with 20 Gy achieved the peak value, suggesting that higher mutagenic potential may occur at 20 Gy. Simultaneously, increase of SOD activity was induced by heavy ions to counteract ROS accumulation. On the other hand, higher doses at 40 and 80 Gy inhibited wheat growth and survival in comparison with the control, and reversely lower doses at 10 or 20 Gy stimulated wheat growth and survival. In conclusion, the above observations imply that a dose range of 20~40 Gy is likely promised for wheat mutation breeding. 12 C 6+ ion Wheat Reactive oxygen species Plant growth Plant survival References Guan M , Li X . Acta Agrom Sin , 2006 , 32 : 878 - 883 . Acta Agrom Sin Tang C X , Liu Z F , Shi J G , et al . Nucl Tech (in Chinese) , 2005 , 28 : 30 - 33 . Nucl Tech (in Chinese) Mei M , Deng H , Lu Y , et al . Adv Space Res , 1994 , 10 : 363 - 372 . Adv Space Res Dong X C , Li W J , He J Y , et al . J Radiat Res Process (in Chinese) , 2007 , 6 : 359 - 362 . J Radiat Res Process (in Chinese) Dong X C , Li W J , Yu L X , et al . J Radiat Res Process (in Chinese) , 2007 , 1 : 62 - 64 . J Radiat Res Process (in Chinese) Al-Rumaih M M , Al-Rumaih M M , American J Environ Sci , 2008 , 4 : 151 - 156 . American J Environ Sci Pauly N , Pucciariello C , Mandon K , et al . J Exp Bot , 2006 , 57 : 1769 - 1776 . J Exp Bot Carol R J , Dolan L . J Exp Bot , 2006 , 57 : 1829 - 1834 . J Exp Bot Wong H L , Sakamoto T , Kawasaki T , et al . Plant Physiol , 2004 , 55 : 1447 - 1456 . Plant Physiol del Rio L A , Corpas F J , Sandalio L M , et al . J Exp Bot , 2002 , 53 : 1255 - 1272 . J Exp Bot Veljovic-Jovanovic S , Noctor C , Foyer C H , Plant Physiol Biochem , 2002 , 40 : 501 - 507 . Plant Physiol Biochem Elstner E F , Heupel A . Anal Biochem , 1976 , 70 : 616 - 620 . Anal Biochem Hodges D M , Delong J M , Forney C F , et al . Planta , 1999 , 207 : 604 - 611 . Planta Beyer W F , Fridovich L . Anal Biochem , 1987 , 161 : 559 - 566 . Anal Biochem Bradford M M . Anal Biochem , 1976 , 72 : 248 - 254 . Anal Biochem Valavanidis A , Vlahogianni T , Dassenakis M , et al . Ecotoxicol Environ Saf , 2006 , 64 : 178 - 189 . Ecotoxicol Environ Saf Pickert M , Gartenbach K E , Kranz A R . Adv Space Res , 1992 , 12 : 69 - 72 . Adv Space Res Muthukumaran S , SudheerA R , Menon V P , et al . Toxicology , 2008 , 243 : 207 - 215 . Toxicology Sevgiler Y , Piner P , Durmaz H , et al . Pesti Biochem Physiol , 2007 , 87 : 248 - 254 . Pesti Biochem Physiol Yamaguchi H , Morishita T , Degi K , et al . Plant Mutat Rep , 2006 , 1 : 25 - 27 . Plant Mutat Rep 10.1016/S1001-8042(08)60039-1 Q691.5 2008-03-05 2008-06-20 2021-01-19 2008 Nuclear Science and Techniques 03 19