Study on parameters of L-[1-<sup>13</sup>C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease

Wei-Li YAN; Xiang-Tong LIN; Yi-Bin JIANG; Su SUN; Da-Yu SUN

Your Location：

Home >

Browse articles >

Study on parameters of L-[1-¹³C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE | Updated：2021-01-29

- Study on parameters of L-[1-¹³C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease
- Nuclear Science and Techniques Vol. 16, Issue 5, (2005)
- Affiliations：
  
  1.Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai 200040
  2.Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040
- Author bio：
  
  Address for correspondence: Department of Gastroenterology, Huashan Hospital, Fudan University, 12 Wurumuqi (M) Road, Shanghai 200040, China; Tel.: 86-021-54030442; Fax: 86-027-62489999 ex 6330; E-mail: sdy385@yahoo.com.cn
  
  A
  d
  d
  r
  e
  s
  s
  
  f
  o
  r
  
  c
  o
  r
  r
  e
  s
  p
  o
  n
  d
  e
  n
  c
  e
  :
  
  D
  e
  p
  a
  r
  t
  m
  e
  n
  t
  
  o
  f
  
  G
  a
  s
  t
  r
  o
  e
  n
  t
  e
  r
  o
  l
  o
  g
  y
  ,
  
  H
  u
  a
  s
  h
  a
  n
  
  H
  o
  s
  p
  i
  t
  a
  l
  ,
  
  F
  u
  d
  a
  n
  
  U
  n
  i
  v
  e
  r
  s
  i
  t
  y
  ,
  
  1
  2
  
  W
  u
  r
  u
  m
  u
  q
  i
  
  (
  M
  )
  
  R
  o
  a
  d
  ,
  
  S
  h
  a
  n
  g
  h
  a
  i
  
  2
  0
  0
  0
  4
  0
  ,
  
  C
  h
  i
  n
  a
  ;
  
  T
  e
  l
  .
  :
  
  8
  6
  -
  0
  2
  1
  -
  5
  4
  0
  3
  0
  4
  4
  2
  ;
  
  F
  a
  x
  :
  
  8
  6
  -
  0
  2
  7
  -
  6
  2
  4
  8
  9
  9
  9
  9
  
  e
  x
  
  6
  3
  3
  0
  ;
  
  E
  -
  m
  a
  i
  l
  :
  
  s
  d
  y
  3
  8
  5
  @
  y
  a
  h
  o
  o
  .
  c
  o
  m
  .
  c
  n
- Funds：
- DOI： CLC： R817.1
- Published：01 October 2005，
  
  Received：28 March 2005，
Scan for full text
Cite this article
Wei-Li YAN, Xiang-Tong LIN, Yi-Bin JIANG, et al. Study on parameters of L-[1-¹³C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease. [J]. Nuclear Science and Techniques 16(5):293-298(2005)
DOI：

Wei-Li YAN, Xiang-Tong LIN, Yi-Bin JIANG, et al. Study on parameters of L-[1-¹³C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease. [J]. Nuclear Science and Techniques 16(5):293-298(2005) DOI：

Study on parameters of L-[1- 13 C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease 1 2 first-author 1 2 2 2 corresp Address for correspondence: Department of Gastroenterology, Huashan Hospital, Fudan University, 12 Wurumuqi (M) Road, Shanghai 200040, China; Tel.: 86-021-54030442; Fax: 86-027-62489999 ex 6330; E-mail: sdy385@yahoo.com.cn Address for correspondence: Department of Gastroenterology, Huashan Hospital, Fudan University, 12 Wurumuqi (M) Road, Shanghai 200040, China; Tel.: 86-021-54030442; Fax: 86-027-62489999 ex 6330; E-mail: sdy385@yahoo.com.cn sdy385@yahoo.com.cn Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai 200040 Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai 200040 Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040 Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200040 The aims of this study are to investigate the feasibility and validity of the L-[1- 13 C] phenylalanine breath test ( 13 C-PheBT) which has been used to measure hepatocyte functional capacity in hepatitis B virus-related liver disease patients and to propose validity parameters of the test in 12 healthy volunteer, 8 chronic hepatitis and 26 liver cirrhotic patients. 100mg/body nonradiative L-[1- 13 C] phenylalanine ( 13 C-Phe) was administered orally to all subjects. Breath samples were taken before and different intervals within 360 min after administration. The 13 CO 2 / 12 CO 2 enrichment was assessed by isotope ratio mass spectrometer. The parameter percentage 13 C excretion rate 13 CER t (% 13 C dose/h) all peaked within 10-30 min after oral 13 C-Phe application. The parameters such as maximum value of 13 C excretion rate, 13 CER max (% 13 C dose/h) (controls: 18.0±3.3; Child A: 11.0±3.8; Child B: 5.0±0.5; Child C: 3.6±1.2), 13 C excretion rate at 30min, 13 CER 30 (% dose/h) (controls: 11. 9±2.1; Child A: 8.1±0.4; Child B: 6.1±0.9; Child C: 3.2±1.2), 13 C cumulative excretion of first 60 min, 13 Ccum 60 (% 13 C dose) (controls: 9.3±1.4; Child A: 6.6±0.7; Child B: 4.1±0.3; Child C: 2.6±0.9) and half time of 13 C excretion rate, T 1/2 (minutes) (controls: 40.4±4.4; chronic hepatitis: 53.4±4.4; Child A: 59.8±4.5; Child B: 102.0±17.3; Child C: 212.1±87.9) were effective indexes which could be employed to stage hepatocyte impairment and liver functional reserve of advanced HBV-related cirrhotic patients (i.e. healthy subjects, Child A, B, C); T 1/2 was also useful for distinguishing mild HBV-related liver injure. Breath test Cirrhosis Hepatitis Phenylalanine Parameters References Pearl R , Clowes G J , Bosari S , et al . Arch Surg , 1987 , 122 : 468 - 473 Arch Surg Jr Clowes G J , McDermott W , Williams L , et al . Surgery , 1984 , 96 : 675 - 685 Surgery Herold C , Regn S , Ganslmayer M , et al . Dig Dis Sci , 2002 , 47 : 2669 - 2673 Dig Dis Sci Giannini E , Botta F , Borro P , et al . Am J Gastroenterol , 2003 , 98 : 2516 - 2520 Am J Gastroenterol Armuzzi A , Candelli M , Zocco M , et al . Aliment Pharmacol Ther , 2002 , 16 : 1977 - 1996 Aliment Pharmacol Ther Slater C , Preston T , Weaver L . Rapid Commun Mass Spectrom , 2001 , 15 : 1270 - 1273 Rapid Commun Mass Spectrom Schneider J , Schoeller D , Nemchausky B , et al . Clin Chim Acta , 1978 , 84 : 153 - 162 Clin Chim Acta Shreeve W , Shoop J , Ott D , et al . Gastroenterology , 1976 , 71 : 98 - 101 Gastroenterology Wang S Z . Molecular Nuclear Medicine (in Chinese) . Beijing : Peking Union Medical College Press , 2001 : 375 - 377 Molecular Nuclear Medicine (in Chinese) Lam T K , Leung D T . N Engl J Med , 1988 , 28 : 318 - 1130 N Engl J Med Gehan E , George S . Cancer Chemothe Rep , 1970 , 54 : 225 - 235 Cancer Chemothe Rep Haycock G , Schwartz G , Wisotsky D . The Journal of Pediatrics , 1978 , 93 : 62 - 66 The Journal of Pediatrics Cortiella J , Marchini J , Branch S , et al . Am J Clin Nutr , 1992 , 56 : 517 - 525 Am J Clin Nutr Oxford Textbook of Clinical Hepatology . New York: Oxford University Press , 1991 : 157 - 174 New York: Oxford University Press Levine R , Conn H . J Clin Invest , 1967 , 46 : 2012 - 2020 J Clin Invest Heberer M , Talke H , Maier K , et al . Klin Wochenschr , 1980 , 58 : 1189 - 1196 Klin Wochenschr O'Keefe S , Abraham R , El-Zayadi A , et al . Gastroenterology , 1981 , 81 : 1017 - 1024 Gastroenterology Burke P , Stack J , Wagner D , et al . Am J Surg , 1997 , 173 : 270 - 274 Am J Surg Kobayashi T , Kubota K , Imamura H , et al . Eur J Clin Invest , 2001 , 31 : 356 - 361 Eur J Clin Invest Ito A , Kohno T , Hosoi I , et al . Digestion , 2001 , 63 : 130 - 138 Digestion Ishii Y , Suzuki S , Kohno T , et al . J Surg Res , 2003 , 112 : 38 - 42 J Surg Res R817.1 Supported by a grant from the Science and Technology Foundation of Shanghai Health Board (No.01406) 2005-03-28 2005-10-01 2021-01-19 2005 Nuclear Science and Techniques 05 16

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

MÖSSBAUER STUDIES OF HEMOGLOBIN AND THE IMMUNE FUNCTION OF RBC OF THE PATIENTS WITH HEPATIC DISEASES

Related Author

Related Institution

The Naval Medical Research Institute

Nanjing University

⁰

Study on parameters of L-[1-13C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease

Study on parameters of L-[1-¹³C]phenylalanine breath test for quantitative assessment of liver function in healthy subjects and patients with hepatitis B virus-related liver disease