Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody

Preparation, characterization and radiolabelling of antigranulocyte monoclonal antibody 1 first-author 1 1 1 Huashan Hospital, Shanghai Medical University, Shanghai 200040 Huashan Hospital, Shanghai Medical University, Shanghai 200040 The human granulocytes were isolated. Using hybridoma techniques, a hybridoma cell line (HSN) producing monoclonal antibody (McAb) against human granulocyte was obtained. The antibody belonged to IgG1 subclass. It was confirmed by immunohistochemical tests that HSN reacted selectively not only with human granulocytes, but also with their bone marrow precursors. Whereas human lymphocytes and red blood cells retained negative in the tests. No cross-reaction was observed with the peripheral blood cells in other animals. Its affinity constant was 5.7×10 8 L/mol, and the number of epitopes per granulocyte was 4.7×10 5 . Monoclonal antibody displayed no loss of immunoreactivity after labelled with 99m Tc. Monoclonal antibody Human granulocytes Technetium-99m labelling Hybridoma References Buchegger F , Schreyer M. Carrel S et al . Int J Cancer , 1984 , 33 : 643 Int J Cancer Locher J T , Seybold K , Andres R Y et al . Nucl Med Commun , 1986 , 7 : 659 Nucl Med Commun Joseph K , Hoffken H , Bosslet K et al . Eur J Nucl Med , 1988 , 14 : 367 Eur J Nucl Med Becker W , Lauer U , Marienhagen J et al . J Nucl Med , 1992 , 33 : 903 J Nucl Med Collet B , Maros S , Morisan A et al . Nucl Med Biol , 1993 , 20 : 175 Nucl Med Biol Gu Z X. Xue F P , Lin X T . Nucl Tech (in Chinese) , 1994 , 17 : 642 Nucl Tech (in Chinese) Becker W , Goldenberg D M , Wolf F . Semin Nucl Med . 1994 , 24 : 142 Semin Nucl Med Hansen H J. Jones A L , Skarkey R F et al . Cancer Res , 1990 , 50 : 764 Cancer Res Schubiger P A , Hasler P H , Novak-Hofer I et al . Eur J Nucl Med , 1989 . 15 : 605 Eur J Nucl Med Supported by National Natural Science Foundation of China under Contract No. 39370218 and International Atomic Energy Agency under Research Contract No.9466/R0 1999-06-01 1999-11-01 2021-02-05 1999 Nuclear Science and Techniques 4 10