A rapid easy-to-perform solid phase digoxin radioimmunoassay

A rapid easy-to-perform solid phase digoxin radioimmunoassay 1 first-author 1 1 1 Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800 Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800 A solid-phase-radioimmunoassay (SPRIA) for the monitoring of blood digoxin level has been developed, in which a secondary antibody-coated polystyrene tubes are used. This noval method seems to be simple to use and only takes about an half hour. The standard curve is linear from 0.25 to 4 µg/L. The sensitivity of the detection is 0.1 µg/L. Reproducibility studies with 3 control sera of 0.5~2.5 µg/L give intraassay CV < 5% and interassay CV < 10%. The specimens are measured and compared with those of the conventional radioimmunoassay and the values are well correlated ( r =0.96, Y =1.022 X +0.04 µg/L). SPRIA Digoxin Serium References Hunter W M , Greenwood F C . Nature , 1962 , 194 : 495 Nature Greenwood F C , Hunter W M . J Biochem , 1963 , 89 : 114 J Biochem Goodman L S , Gilman A . The pharmacological basis of therapeutics , 7 th ed. New York : the Macmillan , 1985 . The pharmacological basis of therapeutics Anthony W . Therapeutic drug monitoring , 1990 , 12 : 6 Therapeutic drug monitoring 1998-07-15 1998-11-01 2021-02-05 1998 Nuclear Science and Techniques 4 9