Ring chromosome aberrations in human lymphocytes induced by 5

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE

Ring chromosome aberrations in human lymphocytes induced by 5–20 Gy carbon ion irradiation

WANG Zhuanzi，

LI Wenjian，

WANG Biqian，

XING Junzhen

Nuclear Science and Techniques

Vol.21, No.6

pp.362-365

Published in print 20 Dec 2010

DOI：10.13538/j.1001-8042/nst.21.362-365

38900

In this paper, we study the biological response induced by heavy ions irradiation of high dose, human peripheral blood lymphocytes are irradiated in vitro by the carbon ions of LET=35 keV/μm, and the chromosome aberrations at absorbed doses of 0–20 Gy are analyzed by the calyculin A-induced premature chromosome condensation (PCC). The frequencies of PCC-rings at the stage of G2/M-phase increase steeply with radiation doses up to 20 Gy at a rate of 0.017 Gy^–1. The G2-PCC index remains more than 5% up to 15 Gy, and 3% after 20 Gy, this is high enough to score a substantial number of chromosome spreads without dose-rate effect. The results show that the calyculin A-induced PCC technique is suitable for analyzing the chromosome damage induced by carbon ions irradiation of high dose.

Carbon ion beamsHuman lymphocytesPrematurely condensed ring chromosome

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