Application of N-succinimidyl 4-[18F](fluoromethyl) benzoate to protein labeling

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE

Application of N-succinimidyl 4-[¹⁸F](fluoromethyl) benzoate to protein labeling

LI Jun-ling，

WANG Yong-xian，

ZHANG Xiu-li，

ZHOU Wei，

TIAN Hai-bin，

YIN Duan-zhi

Nuclear Science and Techniques

Vol.14, No.1

pp.69-71

Published in print 01 Feb 2003

CLC：Q503

30500

N-succinimidyl 4-[¹⁸F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S¹⁸FMB with IgG including pH of solutions, protein concentration, reaction temperature and time were studied. The optimal labeling conditions were: 0.2mg/mL IgG, pH = 7.8-8.5, 25℃, and reaction time 5min.Under these conditions the yield was about 80%. The ¹⁸F-labeled protein was purified by size exclusion chromatography.

S18FMBIgGProtein labelingSize exclusion chromatography

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