RAPD analysis of alfalfa DNA mutation via N+ implantation

LOW ENERGY ACCELERATOR, RAY TECHNOLOGY AND APPLICATIONS

RAPD analysis of alfalfa DNA mutation via N⁺ implantation

LI Yu-Feng，

HUANG Qun-Ce，

LIANG Yun-Zhang，

YU Zeng-Liang

Nuclear Science and Techniques

Vol.14, No.3

pp.161-163

Published in print 01 Aug 2003

CLC：Q345+.2,Q943

40000

Germination capacity of alfalfa seeds under low energy N⁺ implantation manifests oscillations going down with dose strength. From analyzing alfalfa genome DNA under low energy N⁺ implantation by RAPD (Random Amplified Polymorphous DNA), it is recommended that 30 polymorphic DNA fragments be amplified with 8 primers in total 100 primers, and fluorescence intensity of the identical DNA fragments amplified by RAPD is different between CK and treatments. Number of different polymorphic DNA fragments between treatment and CK via N⁺ implantation manifests going up with dose strength.

AlfalfaN+ implantationGermination capacityRAPD (Random Amplified Polymorphous DNA)

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