Development of a soft X-ray microprobe for single cell radiobiology

SYNCHROTRON TECHNOLOGY AND APPLICATIONS

Development of a soft X-ray microprobe for single cell radiobiology

CHEN Liang，

YAN Jingwen，

JIANG Shiping，

YU Yang

Nuclear Science and Techniques

Vol.20, No.2

pp.82-86

Published in print 20 Apr 2009

DOI：10.13538/j.1001-8042/nst.20.82-86

50600

An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells. The facility used synchrotron radiation as soft X-ray source. A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined doses. The diameter of microprobe at the target position was about 2 µm by scanning a knife-edge with an AXUV photo diode. The fluxes of soft X-rays at 516.7 eV (2.4 nm) were about 5.4×10⁴ photons/s.100mA measured with the photo diode. The absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA. A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological investigations. The soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time.

Synchrotron radiationSoft X-raysMicroprobeRadiation biology

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