Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

LOW ENERGY ACCELERATOR AND RADIATION APPLICATIONS

Effects of nitrogen ion irradiation on endoglucanase activity and gene mutation of Bacillus subtilis Bac01

LÜ Jie，

MAO Peihong，

JIN Xiang，

YU Long，

YING Hanjie

Nuclear Science and Techniques

Vol.20, No.5

pp.271-276

Published in print 20 Oct 2009

DOI：10.13538/j.1001-8042/nst.20.271-276

32700

Bacillus subtilis Bac01 was mutated by 15 keV N⁺ ions of 1.5×10¹⁶ cm^-2. The mutant strain Bac11 with high yield of endoglucanase was isolated using carboxymethylcellulose sodium and congo red indicative plates. It exhibited higher endoglucanase activitiy (381.89IU) than the original strain Bac01 (93.33IU). Two 1,500 bp endoglucanase gene fragments were obtained with PCR amplification from B. subtilis Bac01 and mutant strain Bac11. BLAST comparison result indicated that 10 nucleotides mutated. Bioinformatics methods were used to analyze the two predicted amino acid sequences, and it was found that 5 amino acid residues changed, being all in the cellulose-binding domain of endoglucanase.

Ion irradiationBacillus subtilisEndo-1;4-β-D-glucanaseActivityGene mutation

References

[1]

Gao P J. Prog Natl Sci, 2003, 13(1): 21-29.

[2]

Sun Y, Jin X, Mao P H. Biotechnology, 2005, 15(3): 87-89.

[3]

Muller H J. Science, 1927, 66: 84-87.

[4]

Gu S B, Yao J M, Yuan Q P, et al. Appl Microbiol Biotechnol, 2006, 72: 456-461.

[5]

Yu Z L, He J J, Deng J G, et al. Anhui Agricult Sci, 1989, 28: 12-16

[6]

Vilaithonga T, Yu L D, Apavatjrutb P, et al. Phys Chem, 2004, 71: 927-935.

[7]

Yu L, Yu Z L. High Technol Lett, 2002, 12: 41-46.

[8]

Yuan C L, Yao J M, Wang J, et al. J Radiat Res Process, 2003, 21: 237-242.

[9]

Wu L F, Yu Z L. Radiat Environ Biophys, 2001, 40: 53-57.

[10]

Wu Y J, Wang X D, Liu G F, et al. J Nucl Agric Sci, 1990, 11: 154-156.

[11]

Feng H Y, Yu Z L, Chu P K. Mat Sci Eng R, 2006, 54: 49-120.

[12]

Jung H C, Park J H, Park S H, et al. Enzyme Microb Technol, 1998, 22(3): 48-54.

[13]

Liu M, Wang J, Liu J, Yao , et al. Ann. Microbiol, 2006, 56: 41-45.

[14]

Lü J, Jin X, Mao P H, et al. Appl Biochem Biotechnol, DOI 10.1007/s12010-009-8597-9.

[15]

Wang Y J, Yuan H, Wang J, et al. Bioresour Technol, 2009, 100: 345-349.

[16]

Shao C L, Yu Z L. Radiat Phys Chem, 1994, 44(6): 652-654.

[17]

Huang W D, Yu Z L, Jiang X Y. Radiat Phys Chem, 1996, 48(3): 319-323.

[18]

Shao C L, Yu Z L. Radiat Phys Chem, 1997b, 50(6): 595-599.

[19]

Hilden L, Johansson G, Biotechnol Lett, 2004, 26: 1683-1693.

[20]

Takashima S, Ohno M, Hidaka M, et al. FEBS Lett, 2007, 581: 5891-5896.